User talk:Ykgbmn/sandbox

Peer Review
In the preface to the Regulation section, the first sentence might cause confusion. You mention four different proteins but in the following sentence refer to “both of these proteins” without specifying which two you are talking about. As well in the preface, you talk about how the Gvp proteins and environment regulate gas vesicle formation separately. However, in the sources you cite, it seems that the proteins and environment are linked. The article you cited says that the change in environmental conditions changes the expression of these Gvp proteins which in turn regulates the amount of gas vesicles. As well, I feel like the subsection of Ultrasonic algaecide is misplaced. The other subsections describe how the bacteria or archaea themselves change the number of gas vesicles formed in response to changes in external conditions. Ultrasonic algaecide is describing a method of rupturing gas vesicles done by humans, not by the organism itself. I think I would either delete or move the whole subsection. To move it, I think you could make a new subsection titled “Regulation by Humans” and discuss methods that humans use to control bacterial and archaeal growth by regulating gas vesicles. Also, if you were to keep the Ultrasonic algaecide subsection, you would need a new citation for it as the citation for the ultrasonic algaecide subsection is not a reliable source. It is cited to a PowerPoint on how to control growth of aquatic weeds. Furthermore, in the first sentence of the Oxygen Concentration subsection, I think that the lack of oxygen leading to less gas vesicle formation shouldn’t be generalized to all organisms but only to what was stated in the source which are halophilic Archaea.

Cheng Xi (talk) 05:34, 9 November 2017 (UTC)

Microbial Loop Critique
There are certain phrases in this article that may imply bias, such as "It has become clear", "mainly controlled", and "dominates".

In the "History" section, the "key roles of microbes in ocean productivity" from Larry Pomeroy's paper are not summarized, and their contributions to the history of the microbial loop are not explained. According to the paper itself, there is evidence that marine microbes release dissolved organic matter that can be readily used for anabolic processes by other organisms. But without including this in the article, this sentence seems out of place. The following sentence is also unnecessary, as the fact that the term "microbial loop" was introduced in Azam's article is more relevant to the history of the microbial loop rather than the fact that it was published by a "panel of top scientists".

Contrary to the first sentence of the "Factors" section, Taylor and Joint concluded that bacteria are NOT important factors in microbial loop efficiency, as little organic matter released by bacteria is taken to higher trophic levels. This is supported by Ducklow's findings. The section also seems to be outdated, as new research suggests that light plays a role in certain oligotrophic food webs by mitigating mixotrophic bacterivory.

Lastly, I am confused about the use of both "References" and "Bibliography". Citations in the "Bibliography" section can be found online, but they have no hyperlinks in this article. These two sections should be merged into a single "References" section, with hyperlinks attached to the articles.

Gas vesicle evaluation
The topic of gas vesicles is notable; gas vesicle protein genes (gvp) can be found in many bacteria such as cyanobacteria, halophilic archaea, and Bacillus megaterium. A variety of articles discussed different aspects of gas vesicles, like the contributions of proteins GvpA, GvpC and additional accessory proteins to forming the vesicle wall. Other articles studied the regulation of gvp, and how it is affected by a higher pH and contrasting regulators GvpD and GvpE. Additional factors inhibit vesicle formation such as the presence glucose and the lack of oxygen. Lastly, one article discussed the negative impact on enetrobacteria buoyancy, vesicle formation, antibiotic production and cell division from overproducing certain vesicle proteins.

The article, as a whole, is underdeveloped. The “Function” section, in particular, is very short. It is one sentence long. The sentence summarizes only two of the functions of gas vesicles - cell buoyancy modulation and its positioning along the water column, but the summary has no depth. Also, the citation used is from a relatively older source. More recent articles have been published. The "Growth" section is a little better - it is two sentences long. However, there are no citations to either statement. Furthermore, contrary to the second sentence, there is evidence that GvpC controls the diameter and angle of the conical ends of vesicles in Halobacterium salinarium.

Recent research has focused on factors regulating gas vesicle formation. Hence, I plan on adding a new section that discusses the various factors affecting regulation of gas vesicle formation. Some of these factors have their own method of regulation at the molecular level. For example, increased extracellular pH levels decreased decay of gvp transcripts of certain Microcystis species, leading to upregulation of Gvp proteins. In contrast, decreasing extracellular oxygen led to a decreased number of vesicles, gvp transcripts and Gvp proteins in Haloferax mediterranei and ''Hfx. volcanii.'' Other factors cooperatively affected vesicle regulation: one study found that vesicle production in cyanobacteria decreased at high light intensity by accumulating carbohydrates. Light intensity was also found to increase turgor pressure in Anabaena flos-aquae, leading to vesicle collapse.