Wikipedia:USEP/Courses/JHU MolBio Ogg SP14/Group 82G

Article selection rationale

Team 82G (Tyler McKenney and Mike McGinnis) decided to select Northwestern Blot for our article topic. In our down-select process, we first looked at selecting a stub article instead of a more developed article. This particular article does not exist, and we understand that truly starting from scratch may actually entail more work than working on an article that is further developed. Our rationale for this approach is that this provides more of a blank slate and allows for more freedom with regard to organization and format. From there, we then selected from a few different stub articles. The other articles that were considered were, Branch Migration, Acetyltransferase, Paraptosis, Ubiquitin-conjugating enzyme, Bacteriolytic and Equine arteritis virus leader TRS hairpin (LTH). We settled in on Northwestern Blot, as the topic is of interest to both of us and is somewhat relevant from a professional perspective. Tyler has relevant experience with gel electrophoresis and Western Blotting, and we felt that this personal knowledge will add considerable value to our project and ultimately to the article. In addition, Mike has access to several respected purification scientists, and can leverage these relationships if needed. From a technical perspective, given this article does not exist currently; we feel it will be a useful contribution to our industry and potentially of value to many scientists as a reference.

Unit 8 Progress Report


 * As the article did not exist, all current content was added during Unit 8.
 * Several relevant internal links to other Wikipedia articles were made.
 * 5 external references were linked in the contributions made.
 * Attempted to make content somewhat consistent with existing information already created for similar techniques (other blotting techniques)

Unit 10 Progress Report

We have made good progress on our article and just noticed today that our article has been upgraded to Start Class. Below are some specifics regarding our progress.


 * Feedback received from our initial reviewers has been addressed and all reviewers were responded to.
 * An image was added and a link was provided to an article that contains additional relevant images. This was to avoid any potential copyright issues as no copyright-free images of a Northwestern blot have been located thus far.
 * Additional citations were added.
 * Additional content was added in most sections and existing content was reworked for better presentation.
 * An advantages/disadvantages section was added.
 * Links to protocols were added
 * Image of Southern was added

Unit 12 Progress Report We have made a lot of minor revisions based on the feedback received. We've responded to all reviewers to acknowledge their comments and to also indicate our plan. We plan on adding a process flow in the technique specifics section, but need more time to create this. Below are some specifics regarding our progress during this last period.
 * Additional images have been added. An image of a gel electrophoresis device was added, as was a generic western blot image.
 * A spell check and grammar check was conducted to clean up all sections.
 * The Technique Specifics and Applications sections were revised for better flow.
 * Additional content was added in the Origin section regarding gel electrophoresis.
 * All citations were removed from the intro section, per feedback from multiple reviewers.
 * Additional references were added to the technique specifics, applications, and advantages and disadvantages sections.
 * Additional wiki-links were added.

Unit 14 Progress Report We have continued to refine our article, and have made targeted changes using the feedback from the reviewers. The specifics are highlighted below:
 * A process flow was added to describe and allow readers to better visualize the northwestern blotting process.
 * Content was added to the advantages and disadvantages section, most notably more disadvantages were added.
 * The layout of the images was revised to make better use of space and to improve the visual appearence of the article.
 * The lead section was revised for clarity.
 * Additional references were added to the technique specifics section.
 * The categories at the bottom of the page were fixed so the links all work correctly.