Wikipedia:United States Education Program/Courses/JHU MolBio Ogg 2013/Group 82F

Group 82F
This is the Wikipedia page for 410.602 Molecular Biology, Spring, 2013, group 82F. This group will be working on the article Eukaryotic DNA replication.

Use the talk page here to collaborate as a group, when learning to use and navigate Wikipedia, assessing articles, or for any other topic.

Use this page (not the talk page) for article assessments; rationale for selecting an article; etc.

Please create a new section here for each of those assignments.

Ribosomal RNA
This article contains information that is difficult to understand and not very concise. The article also doesn't have many inline citations or very many references. There needs to be added information about the translation process or link the topic to another Wikipedia page on translation. There also need to be more information about the importance of rRNA within the cells. The article seems to focus on the molecular structure of ribosomal RNA and not the functionality or uses of rRNA within the cell.

Ribosomal RNA stub page


 * 1) Ribosomal proteins: structure, function, and evolution
 * 2) Evolutionary origin of RNA editing
 * 3) Structure and Function of the Eukaryotic Ribosome:The Next Frontier

Eukaryotic DNA replication
This article is missing viable information about the mechanism of eukaryotic DNA replication and the proteins that are involved. The involved proteins could be linked to an internal Wikipedia page, but they at least should be mentioned and their purpose explained. The article also focuses too much on the location of DNA replication in eukaryotes and links you to an internal page, DNA replication, for more information. As stated before, the mechanism of eukaryotic DNA replication should be discussed in order to be compared to prokaryotic DNA replication, which this article states "DNA replication in eukaryotes is much more complicated than in prokaryotes, although there are many similar aspects". These similarities could be discussed. The article also doesn't have many inline citations and needs more references.

Eukaryotic DNA replication stub page


 * 1) Balancing eukaryotic replication asymmetry with replication fidelity
 * 2) Eukaryotic lagging strand DNA replication employs a multi-pathway mechanism that protects genome integrity
 * 3) Eukaryotic Lagging Strand DNA Replication Employs a Multipathway Mechanism That Protects Genome Integrity

Nick
The Nick (DNA) stub article is very brief and over simplfied. The article goes into no detail on the enzymes or damage that cause the DNA nicks. There is also a statement regarding the release of torsion in the DNA strand during replication. This could definitely be expanded upon to include why this is important and a description of how nicks facilitate this release. There are also no references or citations at all in the article. This article is classified as high importance on the WikiProject Molecular and Cellular Biology importance scale. The textbook would actually be a great reference for this article as well as the other references listed below.
 * 1) www.ncbi.nlm.nih.gov/pubmed/3474515

Adenosine Diphosphate
The Adenosine diphosphate stub article is listed as high importance on the WikiProject Molecular and Cellular Biology importance scale. The article lacks in citations and references. There also seems to be some incorrect information in the article concerning ADP activation of P2X1 receptors. The article could be expanded to include information on the loss of the phosphate group of ATP that results in ADP. Several statements in the article should include citations and expansion such as the statement that 'single nucleotides (ADP) have the ability to catalyze organic reactions. Other references for this article are listed below.
 * 1) www.ncbi.nlm.nih.gov/pubmed/17961220
 * 2) www.ncbi.nlm.nih.gov/pubmed/17459151
 * 3) www.ncbi.nlm.nih/pmc/articles/PMC2896262
 * 4) onlinelibrary.wiley.com/doi/10.1002/chem.200390109/full

Article selection rationale
Our group has selected the article eukaryotic DNA replication. Our groups’ rationale for selecting the article is to use the rest of the semester to work towards moving the article from a stub class article to either a B or a GA class article. The current article is a stub class article mainly due to the lack of reliable information involving protein assembly of the eukaryotic DNA replication system and how these assemblies assist in the replication of eukaryotic DNA and focuses on DNA replication as it occurs during the cell cycle. This is viable information to have within the article, but there needs to be more information about the different proteins that create the replication complexes. There should also be a section describing how this replication process is performed in the cell, along with images that depict these mechanisms. The presence of more than one replicator should also be mentioned in the article due to the fact that more than one replicator is used to insure that DNA replication is completed for each chromosome. Specifically there could be more expansion regarding the recognition of the origins of replication and pre-RC complex formation as they relate to each other and replication. Also expansion of the topic of the MCM and its components would be helpful as these are mentioned in the article with no detail and is a bit confusing. The protein kinases may be another topic on which to elaborate as they are also important. The article also needs to include more inline citations and more reliable references which will increase the ratings of the article. Within the talk pages for the article, it has been suggested that the article compare eukaryotic DNA replication to prokaryotic replication to provide a good learning tool for readers and promote understanding of the process by comparing the two. The chosen article may need to be rewritten, also stated in the articles talk pages, so that the information can be easily read and understood. There is plenty of additional information that can be added to the article to make it a more reliable source for understanding eukaryotic DNA replication.

Unit 9 progress report

 * An image was created for a visual of the pre-replicative complex and uploaded to Wikicommons, but a more suitable image was found already available on Wikicommons. Also all images that are now on the article page were added by our group.
 * All of our contributions were prose. Multiple sections were added to the article to ensure a better quality of navigation through the information of the article.
 * Added several, if not all, internal links to already existing related Wikipedia pages to all paragraphs.
 * Updated information on already existing list at the bottom of the article page.
 * Added Prokaryotic DNA replication to the "See Also" section.

Unit 14 Final Progress Report
Our group has:
 * Created images using PowerPoint that were needed. These three images are useful for users to better visualize what is being stated in the article. One image is the formation of the pre-replicative complex, the second is a somewhat simplified replisome complex; having main proteins involved pictured. The third image depicts how histones are removed before the replication fork and reassembled after the replication fork.
 * We have also created a master list of main proteins involved in eukaryotic DNA replication and we have added all proteins that are mentioned within the article.
 * We have also added "Processivity" to the "See Also" section of the article. Processivity would be important to add to the article in explaining the process of adding nucleotides by DNA polymerases, but we ran out of time to add some of this information to the article.
 * We have added a substantial amount of information and references to the article. Most references are as recent as we could find. We added a section briefly comparing prokaryotic DNA replication to eukaryotic DNA replication. Within this section, we created a list that can be used to easily compare the major differences between the two processes.
 * We have had wonderful collaborators and commentators that have greatly helped us with this article. Our classmates, Sharkeyr, MonopterusAlbus, DougCovert, and WeberOwl. I would really like to thank Klortho who gave very helpful comments and helped out with many other technical problems that we had towards the beginning of our revision.
 * We believe that our additions/revisions and with the comments from our collaborators, we have enhanced this article so that it is concise and accurate and is able to be used for educational purposes.