Wikipedia:WikiProject Molecular Biology/Molecular and Cell Biology/Help/Archive 3

On the "allele frequency" page
As part of my AP Bio work, I had to find out how allele frequency functions. Yet I've found it hard to understand what exactly is being said in the "allele frequency" page, although this may only be true for some high school level students like me. How this mathematically relates to population frequencies and the Hardy-Weinburg principle would also be helpful... Could someone please explain to me how this operates? Woodland Sun (talk) 07:57, 15 January 2008 (UTC) == leaving regenerating sections wile revising cell articles ==  for people studying in the fields of bioregeneration and stem cell projects, it would be very useful if whom ever is researching the individual cell could take any notes on regenerating chemicals, pros and cons of reactions with the cell with different chemicals and environments, any thing at all that could aid in the search for extended cell life research. thanks Roy Stanley (talk) 13:32, 15 January 2008 (UTC) Retrieved from "http://en.wikipedia.org/wiki/Wikipedia:WikiProject_Molecular_and_Cellular_Biology/Proposals" i thought it nessisary to leave in both proposals and help.. ==Human Genetics== I have been working on the Human Genetics article for a little bit now and was wondering if people would look at it and give back some feedback to help improve it. It would be much appreciated Hrpatel08 :Done! See Talk:Human genetics. My pet article is Genetics and I've been eyeing Human genetics for a long time, I think it needs total rewrite. I suggested a tentative outline there, just as a starting point to think around. Madeleine ✉ ✍ 19:34, 27 March 2008 (UTC) ==Help needed with Action potential==  Hi all,   You might be unaware, but action potential is due to be delisted as a Featured Article very soon, possibly as early as this Saturday, April 5th. A small band of non-scientists is working to save it; perhaps some of you scientists like to help out? The article is still very rough and patchy, as you'll see, but your contributions would be very welcome. Referencing is especially needed; thank you! :) Willow (talk) 22:14, 2 April 2008 (UTC) :So, I'm not too proud to beg.  Are you really going to let a knitter, a mathematician, and two lit-crit humanists walk away with the glory of fixing action potential? ;)  This is not that hard or complicated; it just needs work, but we're running out of time. :P There are a few well-defined sections over there you could just fill in, without too much fuss, methinks. How about the pacemaker potential or the neuromuscular junction? Or better still, how about the cardiac action potential, the muscular action potential, and the plant action potential? Also good would be more about the taxonomic distribution of action potentials; I put down from memory that sponges were the only phylum that didn't get AP's but I can't find a good reference. Please don't forget the references on whatever you write. Thanks, all! :) Willow (talk) 22:31, 3 April 2008 (UTC) == Nociceptor Pain ==  Hello, the current WP:MCOTW is pain and nociception, which has got comlicated... Looks like it may have to be split into pain / pain (sensation) / nociception ... I wouldn't ask. The trouble is splitting pain from physical to metaphysical. If it does split then the nociception part would be in this projects range, but for now there is an article on Nociceptor that hasn't been rated yet. Is it possible for you to assess it, so at least there is another solid anchor to work from, whilst fleshing out the pain problem? Thanks ! LeeVJ (talk) 04:38, 3 April 2008 (UTC) ::The decision was to split so there is now a nociception article, helping to divide the subject better LeeVJ (talk) 19:49, 5 April 2008 (UTC)  == About PDB vs PDB link ==  I noticed these templates have a duplicate functionality. I would like to script a bot to turn all calls to check that all calls make sense, and to turn calls to PDB into PDB link. However, I never ran a bot on Wikipedia before - I can script this easily using pywikipedia, but I want to know the process of getting a bot bit set... Else is there an MCB bot that would like to do this for me? When I log in with pywikipedia (using my regular account) as a test, I see;  WARNING: Your account on wikipedia:en does not have a bot flag. Its edits will be visible in the recent changes and it may get blocked. *** Your username is not listed on Registered bots. *** Please make sure you are allowed to use the robot before actually using it! Which is reassuring at least. In any case, I tested my scripting skill here Beta-lactamase (if you can call it scripting!). python replace.py -transcludes:"PDB" "{{PDB" "{{PDB link"  Am I asking in the right place to get a bot bit? Should I create an account for my bot? --Dan|{{sup|(talk)}} 12:30, 3 April 2008 (UTC) I just ran a check to see which template gets the most usage, I find PDB is used 212 times, and PDB link is used 903 times...   python pagegenerators.py -transcludes:"PDB" | wc -l  python pagegenerators.py -transcludes:"PDB link" | wc -l   So it should be s/\{\{PDB/\{\{PDB link/ ;-) --Dan|{{sup|(talk)}} 12:47, 3 April 2008 (UTC)  AAAAG! I just found {{tl|PDB3}}... which is used 166 times! I'll fix that too if I can. --Dan|{{sup|(talk)}} 12:56, 3 April 2008 (UTC)  AAAAG2! I just found the motherload ... {{tl|PDB2}}, used 2986 times. And don't forget {{tl|PDB enzyme}}, used 4 times!  If I can get a bot bit, I would like to put all of these onto PDB link (assuming there are no format issues). Who maintains the 'Protein Box Bot' that seems to use the {{tl|PDB2}} call? Where can I get a list of the different protein boxes? Cheers, --Dan|{{sup|(talk)}} 13:06, 3 April 2008 (UTC) :Hi Dan, I think that the multiple versions of the PDB template are there because all have slightly different output formats. For example, PDB prefaces the PDB code with a wikilink to PDB, while {{tl|PDB2}} lacks that PDB wikilink. You'll find similar rationale behind {{tl|OMIM}} through {{tl|OMIM5}}. Perhaps what should be done is a systematic update to all the documentation pages so that someone who stumbles on one of the family of templates will know what alternates are available. {{tl|PDB link}} looks pretty much the same as {{tl|PDB3}}, so perhaps there is a bot job there to be done. (Note that procedures regarding bot approval can be found at WP:BOT). Cheers, AndrewGNF (talk) 16:00, 3 April 2008 (UTC) ::I left my comment at Template talk:PDB. Some users wanted to make a link to the "official" PDB site, but others wanted a link to PDBsum which is much superior. So, we need at least two different templates. However I am not sure this matter is of any significance.Biophys (talk) 16:09, 3 April 2008 (UTC) :: I left some comments on Template talk:PDB too. I believe this is a very significant discussion. You say one site is "much superior", I may say another... how can we decide who is right? We need to have a discussion at least, or links to several sites so that people can make up their own minds. Standardizing the link destination is essential. Also, I am not sure if the differences in output are significant ... I'll look in more detail before I do anything to merge template usage. --Dan|{{sup|(talk)}} 20:26, 3 April 2008 (UTC) :::Please do not fix anything before discussing this and reaching consensus with others, as your "fix" (using bot?) can mess up links from several widely used templates. Let's discuss everything at the template talk page.Biophys (talk) 03:15, 4 April 2008 (UTC) ::::OK, I am creating notes here... {{tl|PDB}} --Dan|{{sup|(talk)}} 15:42, 4 April 2008 (UTC) == Appropriateness of gene information box ==  At Histone_methyltransferase, there's a gene information box, but if I'm not mistaken, there are multiple proteins that can be described as histone methyltransferases. Should the article therefore contain gene information boxes for all relevant genes or for none? Seans Potato Business 19:11, 5 April 2008 (UTC) :I think pages on gene families should contain small protein boxes for all member genes if it's a reasonable number (e.g., Inhibin) and wikilinks to the individual gene pages. KEGG lists nine members of this family in humans, so I would support moving that lone protein box to a gene-specific page. AndrewGNF (talk) 20:34, 5 April 2008 (UTC) ==grading== I wrote this page Nucleic acid analogues and I and other people have picked at it for some time. but it has not got a grading. Thanks --Squidonius (talk) 22:21, 10 April 2008 (UTC) See below for template ==New template for large talk pages== {{tn|Unanswered}} Some pages, such as this one have lots of post and it requires some work to see what has been answered or acknowledged. therefore I made the {{tl|Unanswered}} template that can be put above a section allowing one to quickly glimpse what has been answered. For now I will tag mine and any post I am 100% sure is unanswered. If you were waiting for an answer but never got one as the post in somewhere in the middle tag it! please voice any queries or comments in the talk {{twlh|Unanswered}} and not here. Cheers --Squidonius (talk) 14:03, 13 April 2008 (UTC) ==Qiagen== {{tn|Unanswered}} Techniquewise there are some holes around... I found Phenol-chloroform extraction page, which is a protocol straight out of the kit, while I wrote the Guanidinium thiocyanate-phenol-chloroform extraction page which I did not link well (except to trizol) but I give the limited explanation that is present in the litterature. "Qiagen columns" cannot be found only DNA separation by silica adsorption which talks about only one application that are not columns. Anyone know where are those RNeasy columns stored? --Squidonius (talk) 21:31, 17 April 2008 (UTC) == Centrosome ==  The page centrosome is possibly the lest wikified and most ecclectic messy page out there as it is a semi-hot topic as only recently it seems that they are not crucial for mitosis. errors include contradictions, the Dsas4-/- flies mentioned are not really normal (they are uncoordinated (no cilia) but normal developmentally) and "L. elegans" which is... amazing. Could anyone that has read the papers please fix it? Cheers --Squidonius (talk) 14:37, 30 April 2008 (UTC) {{tn|Unanswered}}  ==Identification of enzyme== Hi, in the process of trying to categorise the page Polyadenylate polymerase I ran a search on the following database http://www.ebi.ac.uk/thornton-srv/databases/enzymes/. This came out with a single hit, the enzyme number EC 2.1.1.57. It appears that we already have an article on this enzyme mRNA (nucleoside-2'-O-)-methyltransferase. Can someone with more experience than myself check through and make sure this really is the same enzyme as polyadenylate polymerase before I suggest a merge. Thanks. Ka Faraq Gatri (talk) 18:14, 15 May 2008 (UTC) :No, these are two different enzymes. Polyadenylate polymerase is EC 2.7.7.19, and it is also called Polynucleotide adenylyltransferase or Poly(A) polymerase. Tim Vickers (talk) 18:18, 15 May 2008 (UTC) ::I've merged the pages. Tim Vickers (talk) 18:22, 15 May 2008 (UTC) ==BoTox dose?== НBotulinum Toxin: Fascinated by its cosmetic use. Unable find actual dose level administered: Wikipedia does not give this answer. Ran in to Mouse Units (MU) an unofficial unit of pharmacolocy: a LD50 in mice with a mass of 20 grams that seems to be related but it still does not give me the answer to the actual dose level administered with Botox. Help! User:Mennipus 23:51, 11 June 2008 (UTC) == Cell Biology Wikipedia workshop at ASCB Annual Meeting ==  Dear Molecular & Cell Biology Wikiproject Community,  I'm with the American Society for Cell Biology (ASCB), an 11,000 member professional organization headquartered in Bethesda, MD. At our recent Council meeting, it was decided that we should investigate holding a workshop at our annual meeting (December 13-17, 2008 in San Francisco, CA) that would focus on improving cell biology entries in Wikipedia, the idea being that members would come armed with their laptops and be instructed on how to go to the cell biology entries and work on them. I've been tasked with finding scientist(s) to organize and run this potential session. The selected people would need to be current members of the ASCB, experienced users and annotators of the cell biology Wikipedia entries, and, preferably, be faculty members who teach biology to undergraduate students. Please nominate yourself and/or colleagues who may be interested in running and organizing this workshop. You can contact me at dennist@ascb.org   Regards, Dave (aka, Cellbase)  David L. Ennist, PhD   Director, Digital Resources  The American Society for Cell Biology  8120 Woodmont Avenue, Suite 750  Bethesda, MD 20814-2762  website: http://cellimages.ascb.org/  :See discussion here. Tim Vickers (talk) 19:19, 3 July 2008 (UTC) == Intron == There is a content dispute at Intron (talk) that could use more input. Narayanese (talk) 06:07, 11 August 2008 (UTC) == biology1 ==  explain how a linkage map is created and what information it provides to researchers. ==Please help with the ASCB workshop== As you can read above and on my user page, the American Society for Cell Biology is hosting a workshop this Tuesday, the 16th, to give their scientists a crash course in editing Wikipedia, especiall for creating cell-biology articles. Tim and I are aiming to oblige, but we could benefit from your help! We'd like to find friendly, online Wikipedians who will greet the newbie scientists and offer to help them with their nascent articles. The workshop will run from 12:30-2:30pm local San Francisco time. Tim and I will be speaking until ~1pm, and the participants won't start their user pages until 12:45pm at the earliest. We'll ask them to add the template {{tl|ASCB workshop}} to their user page, which will add them to the {{cl|ASCB 2008 Wikipedia workshop participants}} category. Then you'll be able to see who's participating and welcome them. Other help with categorization, finding/formatting images and references, etc. would be great as well. Here's a list of topics some of the scientists are interested in writing about, in case you wanted to get an overview and maybe read a review paper or two: * nucleus * protein trafficking or presentation on cells * myofibroblasts * Golgi * GW body * pharmaceutical analysis * endothelial progenitor cells * endocytosis * algal eyespots * cell-cell interaction * plasma membrane, lipid rafts * calcium waves * endomembrane system * mitochondria, apoptosis * posttranslational protein modification, specifically arginylation   Thanks very much for your help! Proteins (talk) 12:13, 12 December 2008 (UTC)