KIAA0232
KIAA0232 is a nuclear phosphoserine protein which in humans is encoded by the KIAA0232 gene.
Gene[edit]
KIAA0232 is located at 4p16.1 neighboring TBC1 domain family member 14 and an uncharacterized locus. It has 10 exons which comprise its 4 known transcript variants.[1]
Expression[edit]
KIAA0232 is expressed fairly ubiquitously, but particularly highly in the brain relative to other tissues according to GEO normal tissue expression profiling.[2] Other notable areas of high expression identified by EST profiling include nerves, umbilical cord, and parathyroid.[3]
Homology[edit]
Paralogs[edit]
There are no known paralogs of KIAA0232.
Orthologs[edit]
KIAA0232 is conserved in most animals, including mammals, reptiles, birds, amphibians, insects, and as far back as Trichnella spiralis, a species of nematode. It is not found in fungi, plants, or prokaryotes.[4]
Protein[edit]
The KIAA0232 protein is 1395 amino acids in length with a molecular weight of 154.8kDa. It has higher than average frequencies of serine and glutamic acid residues as well as several multi-serine runs that are evolutionarily conserved. It has an isoelectric point of 4.52.[5][6]
Domains[edit]
KIAA0232 is largely composed of DUF4603.[7]
Subcellular localization[edit]
KIAA0232 is predicted to have nuclear localization.[5]
Post-translational modification[edit]
KIAA0232 is predicted by the tools at ExPASy to be highly phosphorylated, with 15 tyrosine specific sites, 25 threonine specific sites, and 103 serine specific sites.[8]
Interacting proteins[edit]
KIAA0232 is experimentally known to interact with YWHAZ,[9] DYRK1A, and DYRK1B.[10] via two hybrid interaction experiments. YWHAZ is an apoptotic path regulator known to bind to phosphoserine proteins. DYRK1A/B are dual-specificity tyrosine kinases. This corroborates the post-translational modification predictions.
Clinical significance[edit]
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References[edit]
- ^ "KIAA0232 KIAA0232 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-28.
- ^ "NCBI GEO Expression Profiles".
- ^ "EST Profile - Hs.79276". www.ncbi.nlm.nih.gov. Retrieved 2016-05-09.
- ^ "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-05-09.
- ^ a b "SAPS, Statistical Analysis of Protein Sequence". SDSC Biology Workbench.
- ^ "PI, Isoelectric point determination". SDSC Biology Workbench. Archived from the original on 2003-08-11.
- ^ "RCSB Protein Data Bank". Archived from the original on 2012-12-27.
- ^ "NetPhos 2.0 Server". www.cbs.dtu.dk. Retrieved 2016-05-09.
- ^ Bandyopadhyay, Sourav; Chiang, Chih-yuan; Srivastava, Jyoti; Gersten, Merril; White, Suhaila; Bell, Russell; Kurschner, Cornelia; Martin, Christopher H; Smoot, Mike (2010). "A human MAP kinase interactome". Nature Methods. 7 (10): 801–805. doi:10.1038/nmeth.1506. PMC 2967489. PMID 20936779.
- ^ Varjosalo, Markku; Keskitalo, Salla; Van Drogen, Audrey; Nurkkala, Helka; Vichalkovski, Anton; Aebersold, Ruedi; Gstaiger, Matthias (2013-04-25). "The Protein Interaction Landscape of the Human CMGC Kinase Group". Cell Reports. 3 (4): 1306–1320. doi:10.1016/j.celrep.2013.03.027. hdl:20.500.11850/70524. ISSN 2211-1247. PMID 23602568.