1964 in rail transport

April events

 * April 6 - Freight transportation on Alaska Railroad between Fairbanks and Anchorage resumes after repairs from an earthquake that occurred on March 27.
 * April 20 - The Skokie Swift high-speed rapid transit route of the Chicago Transit Authority 'L' system begins service between the Howard Street Terminal in Rogers Park and Dempster Street in Skokie.
 * April 29 - The Keiō Dōbutsuen Line in Japan opens.

June events

 * June 6 - Southern Railway 4501 is taken down to Chattanooga, Tennessee for restoration by the Tennessee Valley Railroad Museum.
 * June 15 - The 2000-series rapid transit cars (2001–2180), built by Pullman-Standard of Chicago, Illinois, are placed in service on the Chicago "L" system. These cars represent the first generation of the Chicago Transit Authority High Performance Family.
 * June 19 - United States President Lyndon B. Johnson presides over the groundbreaking ceremonies for Bay Area Rapid Transit (BART).
 * June 30 - End of regularly scheduled steam locomotive service on the narrow gauge White Pass and Yukon Route.

July events

 * July - The Urban Mass Transportation Act of 1964 becomes law in the United States.

September events

 * September 30 - The Railway Preservation Society of Ireland is formed.

October events

 * October 1
 * The Tōkaidō Shinkansen high-speed route commences operation in Japan; it is the first of many Shinkansen routes to be constructed.
 * Toei Subway Line 1 (present-day Asakusa Line) opens between Shimbashi and Daimon in Tokyo, Japan. This is the fifth extension of the line since it opened in 1960.
 * October 9 - End of District line service to Hounslow on the London Underground.

November events

 * November 5 - Swaziland Railway opened.
 * November 15 - Shin-Sayama Station on what becomes the Seibu Railway's Seibu Shinjuku Line in Sayama, Saitama, Japan, is opened.

December events

 * December 19 - The Elektrische Bahn Stansstad–Engelberg in Switzerland reopens with a connection to the national rail network at Hergiswil and conversion to 15 kV AC railway electrification as the Luzern–Stans–Engelberg railway line.
 * December 23 - In Tokyo, Japan, the Tozai Line begins service between Takadanobaba and Kudanshita.

Unknown date events

 * The Wabash, Nickel Plate Road, Pittsburgh and West Virginia and Akron, Canton and Youngstown railroads are all merged into the Norfolk & Western.
 * Swiss Federal Railways introduces its Re 4/4II series electric locomotives, built by SLM.
 * Double-deck cars introduced on suburban railways in Sydney, Australia.
 * Benjamin Biaggini succeeds Donald Russell as president of the Southern Pacific Company, parent company of the Southern Pacific Railroad.
 * Donald Russell assumes the position of chairman of the Board of Directors of the Southern Pacific Company, a position that was nonexistent since Hale Holden's departure in 1939.
 * ALCO is purchased by the Worthington Corporation.
 * The above-ground portion of Pennsylvania Railroad's Pennsylvania Station in New York City is demolished to make room for Madison Square Gardens, but the tracks remain in use today.
 * Robert A. "Bob" Emerson succeeds Norris Roy Crump as president of Canadian Pacific Railway.