2000–01 Belgian First Division

The 2000–01 season of the Jupiler League began on August 12, 2000, and ended on May 20, 2001. Anderlecht became champions.

Promoted teams
These teams were promoted from the second division at the start of the season:
 * Antwerp (second division champion)
 * Louviéroise (playoff winner)

Relegated teams
These teams were relegated to the second division at the end of the season:
 * Harelbeke
 * KV Mechelen