2016 Advantage Cars Prague Open – Men's singles

The men's singles of the 2016 Advantage Cars Prague Open tournament was played on clay in Prague, Czech Republic. Rogério Dutra Silva was the defending champion but chose not to defend his title.

Santiago Giraldo won the title after defeating Uladzimir Ignatik 6–4, 3–6, 7–6(7–2) in the final.

Seeds
• # 🇸🇰 Martin Kližan (quarterfinals)

• # 🇲🇩 Radu Albot (semifinals)

• # Nikoloz Basilashvili (quarterfinals)

• # 🇸🇰 Jozef Kovalík (second round)

• # 🇪🇸 Daniel Gimeno-Traver (second round)

• # 🇨🇴 Santiago Giraldo (champion)

• # Henri Laaksonen (quarterfinals)

• # 🇦🇷 Facundo Argüello (first round)