2016 Guzzini Challenger – Singles

Mirza Bašić was the defending champion but chose not to participate.

Illya Marchenko won the title after defeating Ilya Ivashka 6–4, 6–4 in the final.

Seeds
• # 🇷🇺 Evgeny Donskoy (semifinals)

• # 🇺🇦 Illya Marchenko (champion)

• # 🇭🇺 Márton Fucsovics (semifinals)

• # 🇨🇴 Eduardo Struvay (first round)

• # 🇧🇪 Ruben Bemelmans (quarterfinals)

• # 🇮🇹 Luca Vanni (quarterfinals)

• # 🇷🇺 Andrey Rublev (second round)

• # 🇧🇦 Aldin Šetkić (second round)