2016 Kobe Challenger – Singles

John Millman was the defending champion but chose not to defend his title.

Chung Hyeon won the title after defeating James Duckworth 6–4, 7–6(7–2) in the final.

Seeds
• # 🇯🇵 Yūichi Sugita (first round)

• # 🇯🇵 Yoshihito Nishioka (quarterfinals)

• # 🇯🇵 Taro Daniel (first round)

• # 🇯🇵 Go Soeda (semifinals)

• # 🇰🇷 Chung Hyeon (champion)

• # 🇰🇷 Lee Duck-hee (semifinals)

• # 🇦🇺 James Duckworth (final)

• # 🇯🇵 Tatsuma Ito (first round)