2016 Open Castilla y León – Men's singles

Evgeny Donskoy was the defending champion but chose not to defend his title.

Luca Vanni won the title after defeating Illya Marchenko 6–4, 3–6, 6–3 in the final.

Seeds
• # 🇪🇸 Nicolás Almagro (quarterfinals)

• # 🇨🇿 Lukáš Rosol (second round)

• # 🇺🇦 Illya Marchenko (final)

• # Marco Chiudinelli (first round)

• # 🇧🇦 Mirza Bašić (semifinals)

• # 🇵🇱 Jerzy Janowicz (first round)

• # 🇫🇷 Kenny de Schepper (quarterfinals)

• # 🇭🇺 Márton Fucsovics (semifinals)