400 meter hurdles at the NCAA Division I Outdoor Track and Field Championships

This is a list of NCAA Division I outdoor champions in the 400 meter hurdles or its imperial equivalent, the 440 yard hurdles. For men, the imperial distance was contested from 1932 to 1975 excepting Olympic years, when the metric distance was contested. Metrication occurred in 1976, so all subsequent championships were at the metric distance. Hand timing was used until 1973, while starting in 1974 fully automatic timing was used. The women's race began in 1982.

Women

 * Key:
 * A=Altitude assisted

Men

 * Key:
 * y=yards
 * A=Altitude assisted