Athletics at the 2020 Summer Paralympics – Women's 400 metres T53

The women's 400 metres T53 event at the 2020 Summer Paralympics in Tokyo, took place on 2 September 2021.

Records
Prior to the competition, the existing records were as follows:

Heats
Heat 1 took place on 2 September 2021, at 12:10:

Heat 2 took place on 2 September 2021, at 12:17:

Final
The final took place on 2 September, at 19:30: