Athletics at the 2020 Summer Paralympics – Women's 400 metres T54

The women's 400 metres T54 event at the 2020 Summer Paralympics in Tokyo, took place on 2 September 2021.

Records
Prior to the competition, the existing records were as follows:

Heats
Heat 1 took place on 2 September 2021, at 12:42:

Heat 2 took place on 2 September 2021, at 12:49:

Final
The final took place on 2 September, at 19:40: