Otago University AFC

Otago University AFC is a semi-professional association football club in Dunedin, New Zealand. The Men's First team competes in the Southern Premier League and has previously competed in the Southern League. The Women's First Team competes in the Women's South Island League.

The club is affiliated with the Otago University Students' Association, and not directly affiliated to the University of Otago in Dunedin, New Zealand; it is one of the oldest clubs associated with the University of Otago, established in 1939. They are based at Logan Park in an area immediately beside the university campus, and play their home fixtures at the nearby Caledonian Ground. The men's first team also participates in the Chatham Cup, New Zealand's premier knockout tournament. The Women's First team competes in the Kate Sheppard Cup. As a member of Southern Football, the club was also affiliated with Southern United of the New Zealand Football Championship.

The clubs best run in the Chatham Cup was in 2014 where they made it to the final 16 before losing 0–1 to Dunedin Technical. However in the 2021 edition, the club made the quarter-finals for the first time, facing North Shore United away.

The club strip is traditionally the Otago region colours of blue and varsity gold, the away strip is traditionally baby blue.