Talk:SYBR Green I

Correct capitilization is SyBR Green if I'm not mistaken--ZayZayEM 06:25, 27 July 2007 (UTC)

Upon reading the short abstract provided by the link, I think that the author of this article emphasized the wrong parts of the study listed as #3, "Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)." The abstract, as I read it, tried to say, "Thus, SYBR Green I stain is a weak mutagen and appears to be much less mutagenic than ethidium bromide." How, then, can it be cytotoxic at lower concentrations than ethidium bromide? I might buy this article, and correct this section later, becuase nowhere else does SyBR green being very cytotoxic (causing cell death) appear on the internet. J1MATHMAN (talk) 19:49, 19 June 2009 (UTC)
 * I was surprised when I read this paper that the abstract seems not to particularly represent the data shown. I have access to the paper and would be happy to send it to you if you email me. Celefin (talk) 20:25, 19 June 2009 (UTC)
 * Ok. I agree with you! My friend found the paper so we will read it and see what it concludes.  Thanks for your reply, and I am reading the paper because I am interested in practical considerations of handling the SYBR green. J1MATHMAN (talk) 15:10, 23 June 2009 (UTC)

disagreement with sigma-aldrich datasheet
There is a slight disagreement between the sigma-aldrich data sheet on SYBR Green I http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Datasheet/s9430dat.Par.0001.File.tmp/s9430dat.pdf

and this article. For example, here it says peak excitation is 488, there it says it is 497. —Preceding unsigned comment added by 173.206.212.40 (talk) 19:25, 27 February 2011 (UTC)

Historic facts
Would be nice to add when SYBR Green I became popular (e. g. first time use should be noted down) and which prior dyes it replaced. (Never mind: the second part is answered via the reference to "ethidium bromide", first part is still missing though.) 2A02:8388:1604:F600:3AD5:47FF:FE18:CC7F (talk) 09:28, 13 December 2021 (UTC)

SYBR Green I versus SYBR Green family
I understand that the most popular variant (or at the least at some point in time) was SYBR Green I. But why the number "I" there? Obviously if there is I aka 1 then there must be more than one I guess. The article mentions this here: "The SYBR family of dyes is produced by Molecular Probes Inc., now owned by Thermo Fisher Scientific." So, a family of dyes. But where can we read up on this? What family? When? Where?

I should explain that a bit. Say that an exam question is as simple as "which dye is commonly used in a real-time PCR?". So, people may answer "SYBR Green" or "SYBR Green I". I assume both answers MAY be correct, but I would also assume that the answer "SYBR Green I" is a bit more specific. What if someone answers "SYBR Green II"? Is this wrong? Perhaps it is wrong, but where to look this up? It would be nice if wikipedia could add some more information in this regard, either in the article here, or in a more general one, e. g. fluorescent dyes, and data where/when/how these were used. Right now I don't know how many SYBR Green variants are in used. Could be 1, 2 or 500 ... 2A02:8388:1604:F600:3AD5:47FF:FE18:CC7F (talk) 10:59, 19 December 2021 (UTC)