2012 Challenger Ciudad de Guayaquil – Singles

Matteo Viola was the defending champion but decided not to participate. Leonardo Mayer won the title after defeating Paolo Lorenzi 6–2, 6–4 in the final.

Seeds
• # 🇦🇷 Leonardo Mayer (champion)

• # 🇮🇹 Paolo Lorenzi (final)

• # 🇪🇸 Rubén Ramírez Hidalgo (semifinals)

• # 🇦🇷 Guido Pella (quarterfinals)

• # 🇦🇷 Martín Alund (semifinals)

• # 🇺🇸 Wayne Odesnik (second round)

• # Frederico Gil (second round)

• # 🇷🇺 Teymuraz Gabashvili (first round)