2013 Fifth Third Bank Tennis Championships – Women's singles

Julia Glushko was the defending champion of the Fifth Third Bank Tennis Championships, having won the event in 2012, but losing in the semifinals to Shelby Rogers in 2013. Rogers went on to win the title, defeating Julie Coin in the final, 6–4, 7–6(7–3).

Seeds
• # 🇯🇵 Misaki Doi (quarterfinals)

• # 🇮🇱 Julia Glushko (semifinals)

• # 🇯🇵 Kurumi Nara (semifinals)

• # 🇺🇸 Shelby Rogers (champion)

• # 🇨🇦 Stéphanie Dubois (first round)

• # 🇺🇸 Grace Min (second round)

• # 🇺🇸 Irina Falconi (second round)

• # Chanel Simmonds (quarterfinals)