2012 Fifth Third Bank Tennis Championships – Women's singles

The 2012 Fifth Third Bank Tennis Championships women's singles was a professional tennis tournament played on outdoor hard courts in Lexington, Kentucky, United States.

Chichi Scholl was the defending champion, but lost in the first round to Bethanie Mattek-Sands.

Julia Glushko won the title, defeating Johanna Konta in the final, 6–3, 6–0.

Seeds
• # 🇦🇺 Olivia Rogowska (first round)

• # 🇯🇵 Erika Sema (first round)

• # 🇮🇹 Karin Knapp (first round)

• # 🇯🇵 Misaki Doi (semifinals)

• # 🇫🇷 Irena Pavlovic (second round)

• # 🇺🇸 Alison Riske (first round)

• # 🇺🇸 Grace Min (first round)

• # 🇺🇸 Madison Keys (semifinals)