2016 Kentucky Bank Tennis Championships – Women's singles

Nao Hibino was the defending champion, but chose to participate in Montreal instead.

Michaëlla Krajicek won the title, defeating Arina Rodionova in the final, 6–0, 2–6, 6–2.

Seeds
• # 🇧🇪 Alison Van Uytvanck (quarterfinals, retired)

• # 🇨🇿 Kristýna Plíšková (withdrew)

• # 🇷🇴 Ana Bogdan (withdrew)

• # 🇨🇳 Zhu Lin (second round)

• # 🇯🇵 Hiroko Kuwata (quarterfinals)

• # 🇺🇸 Grace Min (first round)

• # 🇯🇵 Miyu Kato (first round)

• # 🇷🇺 Anastasia Pivovarova (first round)

• # 🇧🇪 An-Sophie Mestach (second round)