2016 Milo Open Cali – Singles

Fernando Romboli was the defending champion but chose not to participate.

Darian King won the title after defeating Víctor Estrella Burgos 5–7, 6–4, 7–5 in the final.

Seeds
• # 🇩🇴 Víctor Estrella Burgos (final)

• # 🇨🇴 Alejandro González (first round)

• # Gonzalo Lama (first round)

• # 🇨🇴 Eduardo Struvay (second round)

• # 🇧🇷 João Souza (quarterfinals)

• # Marcelo Arévalo (first round)

• # Darian King (champion)

• # 🇦🇷 Juan Ignacio Londero (first round)