2021 Ambato La Gran Ciudad – Singles

This was the first edition of the tournament.

Thiago Agustín Tirante won the title after defeating Juan Pablo Varillas 7–5, 7–5 in the final.

Seeds
• # 🇵🇪 Juan Pablo Varillas (final)

• # 🇸🇰 Andrej Martin (second round)

• # 🇧🇴 Hugo Dellien (withdrew)

• # 🇪🇨 Emilio Gómez (first round)

• # 🇦🇷 Renzo Olivo (first round)

• # 🇦🇷 Juan Pablo Ficovich (semifinals)

• # 🇦🇷 Thiago Agustín Tirante (champion)

• # 🇧🇦 Mirza Bašić (first round)