2021 Zagreb Ladies Open – Singles

Ana Konjuh was the defending champion but chose to compete in Madrid instead.

Anhelina Kalinina won the title, defeating Kamilla Rakhimova in the final, 6–1, 6–3.

Seeds
• # 🇮🇹 Elisabetta Cocciaretto (second round)

• # 🇧🇪 Greet Minnen (first round)

• # 🇵🇱 Katarzyna Kawa (first round)

• # 🇷🇺 Kamilla Rakhimova (final)

• # 🇸🇰 Kristína Kučová (quarterfinals)

• # 🇯🇵 Kurumi Nara (first round)

• # 🇦🇹 Barbara Haas (quarterfinals)

• # 🇷🇸 Olga Danilović (withdrew)