2023 Knoxville Challenger – Singles

Ben Shelton was the defending champion but chose not to defend his title.

Alex Michelsen won the title after defeating Denis Kudla 7–5, 4–6, 6–2 in the final.

Seeds
• # 🇺🇸 Michael Mmoh (first round)

• # 🇺🇸 Aleksandar Kovacevic (first round)

• # 🇺🇸 Alex Michelsen (champion)

• # 🇺🇸 Zachary Svajda (quarterfinals)

• # 🇺🇸 Emilio Nava (semifinals)

• # 🇫🇷 Titouan Droguet (first round)

• # 🇦🇺 Adam Walton (withdrew)

• # 🇨🇳 Shang Juncheng (first round)