List of Ohio railroads

The following railroads operate in the U.S. state of Ohio.

Common freight carriers

 * Akron Barberton Cluster Railway (AB)
 * Ann Arbor Railroad (AA), (Watco)
 * Ashland Railway (ASRY)
 * Ashtabula, Carson and Jefferson Railroad (ACJR)
 * Belpre Industrial, Parkersburg Railroad (BIP)
 * Camp Chase Railway (CAMY)
 * Canadian National Railway (CN) through subsidiaries Bessemer and Lake Erie Railroad (BLE) and Grand Trunk Western Railroad (GTW)
 * Central Railroad of Indiana (CIND) (owned by Genesee & Wyoming (GWI))
 * Chicago, Fort Wayne and Eastern Railroad (CFE) (owned by GWI)
 * Operates Delphos Terminal
 * Cincinnati East Terminal Railway (CET)
 * Cleveland Commercial Railroad (CCRL)
 * Cleveland Harbor Belt Railroad (CHB)
 * Cleveland Works Railway (CWRO)
 * Columbus and Ohio River Rail Road (CUOH) (owned by GWI)
 * CSX Transportation (CSXT) including subsidiary Three Rivers Railway
 * Flats Industrial Railroad (FIR)
 * Grand River Railway (GRRY)
 * Indiana Eastern Railroad (IERR)
 * Indiana Northeastern Railroad (IN)
 * Indiana and Ohio Railway (IORY) (owned by GWI)
 * Operates South Charleston Railroad, West Central Ohio Port Authority
 * Kanawha River Railroad (KNWA) (Watco)
 * Lake Terminal Railroad (LT)
 * Lorain Northern Railroad (LNOR)
 * Mahoning Valley Railway (MVRY) (owned by GWI)
 * Napoleon, Defiance & Western Railroad (NDW), (Pioneer Lines)
 * Newburgh and South Shore Railroad (NSR) (Omnitrax)
 * Norfolk Southern Railway (NS) including subsidiary Cincinnati, New Orleans and Texas Pacific Railway (CNTP)
 * Northern Ohio and Western Railway (NOW)
 * Ohio and National Railway Company (ONRR)
 * Ohi-Rail Corporation (OHIC) (taken over by Mahoning Valley Railway (MVRY))
 * Ohio Central Railroad (OHCR) (owned by GWI)
 * Ohio South Central Railroad (OSCR)
 * Ohio Southern Railroad (OSRR) (owned by GWI)
 * Ohio Terminal Railway (OHIO)
 * Republic N&T Railroad (NTRY)
 * R.J. Corman Railroad/Cleveland Line (RJCL)
 * R.J. Corman Railroad/Western Ohio Lines (RJCW)
 * RSL Railroad (RSL)
 * Warren and Trumbull Railroad (WTRM) (owned by GWI)
 * Wheeling and Lake Erie Railway (WE)
 * Youngstown and Austintown Railroad (YARR) (owned by GWI)
 * Youngstown Belt Railroad (YB) (owned by GWI)
 * Youngstown and Southeastern Railroad (YSRR)
 * Operates Eastern States Railroad and Ohio and Pennsylvania Railroad (OHPA)

Defunct railroads

 * Electric railways
 * Akron, Bedford and Cleveland Railroad
 * Akron and Cuyahoga Falls Rapid Transit Company
 * Blissfield Railroad
 * Canton and Massillon Electric Railway
 * Cincinnati, Dayton and Toledo Traction
 * Cincinnati, Hamilton and Dayton Railway
 * Cincinnati and Lake Erie Railroad
 * Cincinnati Street Railway
 * Cleveland Railway
 * Cleveland, Southwestern & Columbus Railway
 * Columbus, Delaware and Marion Railway
 * Columbus, Marion and Bucyrus Railway
 * Lake Shore Electric Railway
 * Muskingum Electric Railroad (private)
 * Newark and Granville Electric Street Railway
 * Ohio Electric Railway (OE)
 * Sandusky, Milan and Norwalk Electric Railway
 * Shaker Heights Rapid Transit
 * Toledo and Indiana Railway
 * Toledo, Port Clinton and Lakeside Railway
 * Toledo and Western Railway (T&W)
 * Youngstown and Ohio River Railroad