List of Florida railroads

This is a list of railroads operating in the U.S. state of Florida.

Common freight carriers

 * Alabama and Gulf Coast Railway (AGR) (GWI)
 * AN Railway (AN) (GWI)
 * Bay Line Railroad (BAYL) (GWI)
 * CSX Transportation (CSXT)
 * First Coast Railroad (FCRD) (GWI)
 * Florida Central Railroad (FCEN)
 * Florida East Coast Railway (FEC)
 * Florida Gulf & Atlantic Railroad (FGA)
 * Florida Midland Railroad (FMID)
 * Florida Northern Railroad (FNOR)
 * Georgia and Florida Railway (GFRR)
 * Jacksonville Port Terminal Railroad (JXPT) (Watco)
 * Norfolk Southern Railway (NS) through subsidiary Georgia Southern and Florida Railway (GSF)
 * Seminole Gulf Railway (SGLR)
 * South Central Florida Express, Inc. (SCXF)

Private freight carriers

 * CF Industries (CFIZ)
 * Conrad Yelvington Distributors, Inc. (CNYX)
 * The Mosaic Company (IMCX)
 * Orlando Utilities Commission (OUCX)
 * Perdue Farms (PFMX)
 * Port Manatee Railroad (MAUP)
 * Port of Palm Beach District (PPBD)
 * Port of Panama City (PPC)
 * United States Sugar Corporation (USSC)

Passenger carriers

 * Amtrak (AMTK)
 * Brightline (BLFX)
 * Florida Railroad Museum (FGCX)
 * Gold Coast Railroad Museum (GCOX)
 * Jacksonville Skyway
 * Kirby Family Farm Train
 * Miami Metromover and Miami Metrorail
 * Seminole Gulf Railway (SGLR)
 * Serengeti Express (located in Busch Gardens Tampa)
 * Sugar Express (CHOX)
 * SunRail (SRCX)
 * Tradewinds & Atlantic Railroad
 * TECO Line Streetcar
 * Tri-Rail (TRCX)
 * Walt Disney World Monorail and Walt Disney World Railroad
 * Wildlife Express Train

Private carriers

 * Clay Springs and Apopka Railroad
 * Ellaville, West Lake and Jennings Railroad
 * Florida and Georgia Railway
 * Peace River Phosphate Company
 * Pensacola and Andalusia Railroad
 * Starke and New River Railroad
 * Union Cypress Company
 * Winston Lumber Company

Never built

 * Choctawhatchee and Northern Railroad