Nucleic acid methods

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification

 * DNA extraction
 * Phenol–chloroform extraction
 * Minicolumn purification
 * RNA extraction
 * Boom method
 * Synchronous coefficient of drag alteration (SCODA) DNA purification

Quantification

 * Abundance in weight: spectroscopic nucleic acid quantitation
 * Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
 * High-throughput relative abundance: DNA microarray
 * High-throughput absolute abundance: serial analysis of gene expression (SAGE)
 * Size: gel electrophoresis

Synthesis

 * De novo: oligonucleotide synthesis
 * Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)

Kinetics

 * Multi-parametric surface plasmon resonance
 * Dual-polarization interferometry
 * Quartz crystal microbalance with dissipation monitoring (QCM-D)

Gene function

 * RNA interference

Other

 * Bisulfite sequencing
 * DNA sequencing
 * Expression cloning
 * Fluorescence in situ hybridization
 * Lab-on-a-chip
 * Comparison of nucleic acid simulation software
 * Northern blot
 * Nuclear run-on assay
 * Radioactivity in the life sciences
 * Southern blot
 * Differential centrifugation (sucrose gradient)
 * Toeprinting assay
 * Several bioinformatics methods, as seen in list of RNA structure prediction software