Talk:Α-Amylase

Molecular weight
what is the molecular weight? W09110900 (talk) 21:46, 29 November 2008 (UTC)

TOC
The table of contents and general organization into headings seems to be quite messy in this article. I really don't know how to fix this though. Dex Stewart (talk) 18:55, 15 March 2010 (UTC)

First sentence
Alpha-amylase can cleave alpha-1,4 glycosidic linkage. It is named as "alpha" because the products are in alpha-form. Alpha-amylase mainly produce maltose, maltotriose, and maltotetraose. The digestion pattern depends on the source of alpha-amylase. In the other word, the digestion pattern is the characteristics of each alpha-amylase. It rarely produces glucose. After a very long time incubation, little glucose can be detected. However, it is not the main products of alpha-amylolysis. Amy Lin (talk) 03:11, 22 June 2010 (UTC)


 * Thanks for your comment, Amy. I originally added that sentence based on Stedman's Medical Dictionary, which defines α-amylase as "a glucanohydrolase yielding α-glucose and maltose in a random manner from 1,4-α-glucans."  But now that you mention it, I'm pretty sure fungal α-amylases produce predominantly maltose and other short oligosaccharides, not glucose.  Perhaps Stedman's definition is based on human α-amylases, which may not be representative of all α-amylases.  I'll check some sources at uni later in the week (out of time now), but in the mean time you or anyone else is welcome to edit as you see fit.  Adrian J. Hunter(talk•contribs) 04:33, 22 June 2010 (UTC)

Hi Adrian, thanks for the response. I summarize some digestion properties of alpha-amylase here for your reference. As you have mentioned, alpha-amylase from fungal (Aspergillus oryzae, or named as Taka-amylase A) shows a randomness of hydrolysis similar to acid hydrolysis. Others, most of alpha-amylase hydrolyze starch endowise at inner alpha-1,4 linkages. Human pancreatic and salivary alpha-amylase show very similar actions (they are 95% homology in their amino acid sequence). Both produce maltose(G2) preferentially from reducing residues of G4, G5, and G6 and essentially do not act on G3. Porcine pancreatic alpha-amylase, another widely used enzyme, hydrolyzes linear amylose mainly into G2 and G3, and finally into G1 and G2 after prolonged incubation with a large amount of the enzyme. Cereal alpha-amylase hydrolyze amylose mainly to G2 and G4 to G7, then higher oligosaccharides are finally hydrolyzed to G2 and small amount of G1 and G3. Alpha-amylase from Bacillus hydrolyzes amylose to a mixture of G1-G6, preferentially G2, G3 and G6. Hope above information makes things clear :) Amy Lin (talk) 18:16, 22 June 2010 (UTC)


 * Looks good, thanks for the info... Looks like this article has plenty of room for expansion. Do you have references though?  Just URLs or DOIs are enough, as there are tools for creating full references from those (eg this one).  Adrian J. Hunter(talk•contribs) 08:36, 23 June 2010 (UTC)

alpha-amylae in plant seeds with special reference to medicinal plants
can anybody help me in this topic, i —Preceding unsigned comment added by 59.90.154.4 (talk) 04:10, 13 November 2010 (UTC)

Move?

 * The following discussion is an archived discussion of a requested move. Please do not modify it. Subsequent comments should be made in a new section on the talk page or in a move review. No further edits should be made to this section. 

The result of the move request was: moved per request. Uncontested and consistent with other members of Category:Enzymes. Favonian (talk) 19:13, 4 July 2012 (UTC)

Alpha-Amylase → Alpha-amylase – User:Richard Arthur Norton (1958- ) 16:09, 27 June 2012 (UTC)
 * The above discussion is preserved as an archive of a requested move. Please do not modify it. Subsequent comments should be made in a new section on this talk page or in a move review. No further edits should be made to this section.

Inactivation
The test says: "In gastric juice adjusted to pH 3.3, ptyalin was totally inactivated in 20 minutes at 37 °C." and "Ptyalin added to buffer at pH 3.0 underwent complete inactivation in 120 minutes". For a laymen like me this seems contradictory. Could this be explained in the text?163.158.50.4 (talk) 12:56, 30 July 2021 (UTC)