Talk:Fluorescence-lifetime imaging microscopy

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Could someone define all the variables in the second equation in the "Fluorescence lifetimes" section, please.

It would make it much more helpful to those who are new to the topic. Thanks!

-- What about w? —Preceding unsigned comment added by 86.168.237.9 (talk) 09:21, 18 April 2011 (UTC)

speed
How long does it take to capture an image, and what does the time depend on ? - Rod57 (talk) 11:59, 22 October 2012 (UTC)
 * FLIM protocol suggests 60 sec and vary to get about 10^5 photons (per pixel ?) - Rod57 (talk) 12:09, 22 October 2012 (UTC)

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Fluorescence lifetimes wrong
I'm not an expert in this stuff, but I do know my statistical physics. The single exponential decay would be a limiting case only. - If there are parallel observable decays, you would get a sum of exponentials. - If there are serial decays, you can get differences of exponentials (depends on which part of the pathways is fluorescent).

So the statement in this section seems wrong, and should be corrected, or conditions for this approximation mentioned.