Talk:GPR64

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Not got time or the inclination to learn to wikicode at this time, so I'll just post as it stands, all references included and at the limit of knowledge as of December 2010, hopefully someone can sort this all out and post it on the page...

Cheers!

Approved name:		       G protein-coupled receptor 64 [1]

Approved symbol: 		GPR64 [1]

HGNC ID:			HGNC:4516 [1]

Accession number: 		X81892 [1]

Description: 			GPR; Class B; LNB7TM family [3]

Other names: 			CTD-2245E12.1, FLJ00282, HE6, MGC104454, MGC138738, MGC138739, TM7LN2 [2]

Gene name:			GPR64 [2]

Genetic information:		Human 	1017aa Xp22.13 [2]	EGI: 10149 [1] Rat 		1013aa Xq21 [2]	MGI: 2446854 [1] Mouse		1009aa X F4 [2]	RGD: 628618 [1]

Structural information: 	Two subunits; N-terminus large hydrophilic ectodomain with multiple glycosylation sites (~80 kDa with deglycosylation decreasing this to ~70kDa) and a smaller C-terminus hydrophobic endodomain (<40kDa), a conserved cystine rich GPS motif where the protein is 				thought to be cleaved lies between the two domains. In the ectodomain there is a signalling protein, followed by a variable region encoded for by at least 6 exons, then mucin-stalk-like STP sequence. The endodomain contains the 7TMDs and G protein-coupling. [4]

Other receptor homology:	GPS motif and a STP-rich region in the ectodomain shared with other members of the LNB7TM family. [4] BLAST shows particular homology with GPR112 and GPR126.

Tissue distribution:		Human		Epididymus specific [5] (apical membranes of efferent						ductule and proximal epididymal duct epithelia [4]) Mouse		Epididymus, faint signals seen in brain and speen with 						extended exposure [4]

Agonists: 			None known

Physiological importance:	Epididymal function and spermatogenesis through G-protein coupled 				receptor protein signaling pathway [5]

Assocciated pathologies:	Male infertility [6]

Pharmacological importance:	Potential male fertility treatment or male contraceptive pill

Abbreviations:

GPR			g protein-coupled receptor HGNC			human genome organisation gene nomenclature committee aa			amino acid EGI			entrez gene identification MGI			mouse genome database identification RGD			rat genome database identification GPS			g protein-coupled receptor proteolysis site STP			serine/threonine/proline TMD			transmembrane domain BLAST			basic local alignment search tool

References:

1	www.genenames.org [homepage]. HGNC [updated 25/04/05, accessed 18/12/10]. Available from: http://www.genenames.org/data/hgnc_data.php?hgnc_id=4516

2	www.iuphar-db.org [homepage]. IUPHAR [updated 01/07/10, accessed 19/12/10]. Available from: http://www.iuphar-db.org/DATABASE/ObjectDisplayForward? familyId=17&objectId=187

3	Foord et al. IUPHAR. XLVI. G protein coupled receptor list. Pharmacol. Rev. 2005;57:279-288.

4	Obermann et al. HE6, a two-subunit heptahelical receptor associated with apical 	membranes of efferent and epididymal duct epithelia. Molecular Reproduction and 	Development. 2003;64:13-26

5	Osterhoff C, Ivell R, Kirchhoff C. Cloning of a human epididymis-specific mRNA, HE6, 	encoding a novel member of the seven transmembrane-domain receptor superfamily. DNA 	Cell Biol. 1997;16:379-389

6	Davies B, Baumann C, Kirchhoff C, Ivell R, Nubbemeyer R, Habenicht U F, Theuring F, 	Gottwald U. Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation 	and male infertility. Mol Cell Biol. 2004;24:8642-8648 —Preceding unsigned comment added by 109.144.228.11 (talk) 20:59, 24 March 2011 (UTC)