Talk:Glyceraldehyde 3-phosphate dehydrogenase

Formal reaction?
the article states:

The first reaction is the oxidation of glyceraldehyde 3-phosphate at the carbon 1 position (in the diagram it is shown as the 4th carbon from glycolysis), in which an aldehyde is converted into a carboxylic acid (ΔG°'=-50 kJ/mol (-12kcal/mol)) and NAD+ is simultaneously reduced endergonically to NADH.

isnt this reaction just formal?? because this source says exactly that: http://www.chemgapedia.de/vsengine/vlu/vsc/de/ch/8/bc/vlu/stoffwechsel/glykolyse.vlu/Page/vsc/de/ch/5/bc/chemical_pathways/glycolyse/gap/gap.vscml.html --Spannungsquelle (talk) 14:33, 25 August 2015 (UTC)

What does the following sentence say precisely?:
"GAP is concomitantly oxidized to a thioester intermediate using a molecule of water."

Does that mean GAP just gives 1 electron to H2O or does it mean that it is oxidized and therefore gives its electron to some other molecule than water and then is reduced by the water molecule? I suggest this because if i assume GAP just gives one electron to H2O i get one electron to little to form a thioester. --Spannungsquelle (talk) 12:53, 23 August 2015 (UTC)

illustration
I think the equilibrium / enzyme reaction would be easier to 'see' if the GAP (on the left) were inverted to parallel the corresponding part of the image of GAPDH (on the right).--Wikidity (talk) 18:46, 25 August 2011 (UTC)

prokaryote and eukaryote?
is GADPH used by both eukaryotes and prokaryotes?


 * Probably it is, because gycolysis is performed by both euk. and prok. --Christopherlin 15:22, 21 March 2006 (UTC)

And because it has several other essential functions in transcription (grooming), apoptosis (triggering usual or homeostatic cell death), and vesicle formation (already mentioned). --Wikidity (talk) 18:11, 25 August 2011 (UTC)

Additional source
This page http://www.biochem.ucl.ac.uk/bsm/enzymes/ec1/ec02/ec01/ec0012/ explains the EC classification of the enzyme. --Christopherlin 15:22, 21 March 2006 (UTC)

water
"Finally, a molecule of inorganic phosphate attacks the thioester and forms a tetrahedral intermediate, which then collapses to release 1,3-bisphosphoglycerate, a molecule of water, and the thiol group of the enzyme's cysteine residue." - where does the water come from?


 * This was a mistake and has since been corrected. Jasu 13:45, 23 August 2007 (UTC)

Molecular Weight
What's its molecular weight and what is its quaternary structure (monomer, dimer, tetramer... ?)

As I remember, is roughly 37KDa.--Wikidity (talk) 18:14, 25 August 2011 (UTC)
 * Good question. It seems that human GAPDH is a homotetramer, i.e. 4 molecules of GAPDH complexed together. See http://www.expasy.org/uniprot/P04406 Jasu 13:29, 23 August 2007 (UTC)

writing level
is it just me or is this article waaayyyy too technical for an encyclopedia entry? i feel like it should be written more layman-ish and provide references for the extra details. —The preceding unsigned comment was added by 141.213.67.125 (talk) 18:24, 22 February 2007 (UTC).


 * You are right but should more complex information be included for the article to be easy to read? I think the best way forward is to write a widely understood lead section for the article (which I attempted just know) and give more complex information deeper down in the article. In this way, a general reader can understand the topic by reading the leader and specialists may find the article interesting as well. Jasu 13:21, 23 August 2007 (UTC)

extreme makeover
I spend 2-3 hours reworking this article. This included: After this significant extension, I removed the stub markers. Thanks to glycolysis for providing me with energy to write this.
 * extended lead section in plain English
 * more subdivision of the article with headings to facilitate targeted reading
 * new sections on recently discovered functions of GAPDH in transcription, apoptosis, and vesicle formation
 * plus reference section

Jasu 13:25, 23 August 2007 (UTC) Thank you for your contribution. (Let's hear it for sugar industry subsidies;)--Wikidity (talk) 18:50, 25 August 2011 (UTC)

COPI / COPII mixup
"ER to Golgi vesicle shuttling" describes COPII trafficking, but the link takes it to COPI which acts in the opposite direction. Not sure which is correct in this instance, as there's no citation. — Preceding unsigned comment added by 2603:3024:1811:CC00:54D:7B3B:4531:820E (talk) 05:33, 1 November 2016 (UTC)