Talk:Malate–aspartate shuttle

NADH formally carries a hydride anion
The opening paragraph says that NADH carries the "reduction equivalent" of a hydrogen ion. I find it confusing. NADH is the reduced form of NAD+. A hydride anion is the "reduced equivalent" of a proton (or a hydrogen cation). It is clearer to state that NADH formally carries a hydride anion. Since the hydride anion is too strong of a Bronsted base to exist in water, it is carried as part of NADH. --Chibibrain (talk) 06:59, 22 November 2009 (UTC)

cytosol?
The article says malate starts in the cytosol but the diagram shows it in the intermembrane space. How does it get there from the cytosol? —Preceding unsigned comment added by 94.173.122.171 (talk) 16:32, 18 April 2010 (UTC)
 * Unlike the inner membrane, the outer membrane is not a barrier to small metabolites, which freely exchange between cytoplasm and intermembrane space.96.54.32.44 (talk) 01:18, 14 December 2010 (UTC)

This should be explained in the article. I found it very confusing as well!Curase (talk) 03:01, 17 May 2017 (UTC)

GOT1 and MDH1 are cytosolic enzymes. I believe the diagram should be corrected to show the reactions occurring in the cytosol, as is indicated in the "interactive pathway map." — Preceding unsigned comment added by 141.214.17.122 (talk) 17:30, 6 June 2018 (UTC)

Gluconeogenesis
This same pathway is used for reductive carboxyloation of pyruvate to oxaloacetate in the matrix of the mitochondria then transfer of malate and subsequent oxidation back to oxaloacetate during gluconeogenesis. Should a section be included here? ````` — Preceding unsigned comment added by 69.138.157.32 (talk) 17:31, 4 November 2012 (UTC)

Negative Charge on Hydroxy group of Malate?
I do not see why, at physiologic pH that the diagram would show an ionized hydroxyl group on the malate molecule. — Preceding unsigned comment added by 172.78.56.88 (talk) 16:39, 22 February 2017 (UTC)

Assessment comment
Substituted at 22:54, 29 April 2016 (UTC)