Talk:Margaret Robinson

�Overview

Margaret Robinson is an American Molecular Cell Biologist professor and researcher at Cambridge Institute for Medical Research. She received her B.A. in Biology from Smith College in Massachusetts. She attended Harvard University for her Ph.D studying under David Albertini and also Barbara Pearse. She is currently at Cambridge Institute for Medical Research working on coated vesicle proteins. Margaret has received many honors working as a cellular biologist. She was awarded a Wellcome Trust Principal Research Fellowship in 1999 and in 2003 she was appointed Professor of Molecular Cell Biology. She is presently a Fellow of the Academy of Medical Sciences and member of the European Molecular Biology Organization. She was elected part of The Royal Society in 2012. The Wellcome Trust also has funded her research for over 25 years. Her achievements include the discovery of adaptins, which are specific proteins that manage cell-trafficking to ensure the correct cell cargo is transported to the right location. She also discovered different combinations of adaptins, when together with clathrin, form a coat around vesicles that bud from intracellular membranes and act as transporters for protein packages to be distributed in the cell. She also developed the technique “knocksideways,” which inactivates proteins in seconds.

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Early Life

Margaret Robinson never was interested in science until college. She was first exposed about science early in her life from reading about Madam Curie, who she saw as a role model. While enrolled at Smith College, she planned on being an English or theater major. However, due to University requirements, Margaret had to complete an introductory biology course. In that course, Jeanne Powell gave a lecture on cells and showed her students electron micrographs. This is when Margaret really became interested in cellular biology; the complexity of cells intrigued her. After receiving her B.A. in Biology from Smith College, Margaret took a year off because she was unsure about what she wanted to do. She ended up at Harvard Medical School and she learned about coated vesicles while taking a summer class at Woods Hole. Margaret joined David Albertini’s lab, which was a new faculty member at the time, and she was able to research on anything she liked. Due to her inexperience, her research did not go as planned and was nearly kicked out of graduate school. Essentially, Margaret had to stop working on her interest in coated vesicles and work on something closer to what David’s lab was doing. Margaret eventually started a postdoc research with Barbara Pearse. She wanted to find out what was in clathrin-coated vesicles that binds to cargo. She eventually succeeded in purifying components of the coat that were not clathrin and are now known as adaptor proteins. These proteins sit between clathrin, which forms the vesicle’s outer shell and also the vesicle membrane. Continuing, Margaret discovered that there were two different populations of clathrin-coated vesicles, one that uses AP-2 at the plasma membrane and one that uses AP-1 and was associated with intracellular membranes. AP-1 and AP-2 are both heterotetramers with related subunits. They both have two large subunits and the other subunit is closely related in AP-1 and AP-2.

Research

After finishing her postdoc, she was able to start her own lab. Her main focus was to learn more about the AP protein in depth. She had to also work with DNA because in order to characterize the complexes thoroughly, she needed to clone the subunits. Margaret and her lab managed to find another AP complex, AP-3, which interacts with lysosomal membrane proteins such as LAMP1. AP-3 also interacts with tyrosinase, which is a key enzyme in melanin biosynthesis, so AP-3 is important for tyrosinase trafficking to premelanosomes. Margaret now has a lab at Cambridge Institute for Medical Research. She specifically works with coated vesicles. The best-characterized coated vesicels are the clathrin-coated vesicles (CCVs). The coats on CCVs are primarily of clathrin, adaptor protein (AP) complexes, and alternative adaptors. Her working hypothesis is that for each trafficking pathway, there are a number of different adaptors, each of which is recruited independently onto the appropriate membrane. Once on the membrane, the various adaptors would work together to package different types of cargo into the newly forming vesicle. Margaret and her lab uses several approaches to look for novel adaptors and other components of the trafficking machinery, including proteomic analyses of subcellular fractions, genome-wide siRNA library screening, insertional mutagenesis, and a new method they developed for rapidly inactivating proteins, called ‘knocksideways’. Her current projects include establishing the functions of AP-1 and other adaptors in differentiated cells; matching up machinery and cargo proteins; investigating how clathrin and adaptors are hijacked by the HIV-1-encoded protein Nef; determing why mutations in the non-clathrin adaptors AP-4 and AP-5 cause hereditary spastic paraplegia; and exploring the evolution of adaptors. Her laboratory uses many techniques including immunolocalisation at the light and electron microscope levels, subcellular fractionation, protein purification, proteomics, flow cytometry, live cell imaging, and X-ray crystallography.

Impact of Research

Margaret’s research is important because it is fundamental. Every form of eukaryotic life on earth contains coated vesicles and adaptors. Her work is also speculated to play a key role in evolution of eukaryotes form prokaryotes over two billion years ago. Her work also has medical implications. Some adaptors are mutated in certain genetic disorders, and adaptors are frequently exploited by pathogens. For example, the HIV genome encodes a protein called Nef, which is required for the development of AIDS, and which works by hijacking adaptors and using them to modify the surface of the infected cell. Margaret’s work explains how coated vesicles sort cargo but also provides tools that can be used by others to address their own favorite problems. For instance, her newly developed method called knocksideways. Knocksideways gets rid of proteins rapidly. Her technique has found its way into other labs who are also interested in how particular proteins contribute to different stages of cell division.

Featured Publications

Hirst, J., Schlacht, A., Norcott, J. P., Traynor, D., Bloomfield, G., Antrobus, R., Kay, R. R., Dacks, J. B. and Robinson, M. S. Characterization of TSET, and ancient and widespread membrane trafficking complex. eLife 3: e02866 (2014).

Borner, G.H.H., Hein, M.Y., Hirst, J., Edgar, J.R., Mann, M., and Robinson, M.S. Fractionation profiling: a fast and versatile approach for mapping vesicle proteomics and protein-protein interactions. Mol. Biol. Cell. 25: 3178-3194 (2014).

Hirst, J., Borner, G. H. H., Edgar, J., Hein, M. Y., Mann, M., Buchholz, F., Antrobus, R. and Robinson, M. S. Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15. Mol. Biol. Cell 24, 2558–2569 (2013). Kozik, P., Hodson, N. A., Sahlender, D. A., Simecek, N., Soromani, C., Wu, J., Collinson, L. M. and Robinson, M. S. A human genome-wide screen for regulators of clathrin-coated vesicle formation reveals an unexpected role for the V-ATPase. Nature Cell Biol. 15, 50–60 (2013).

Borner, G.H.H., Antrobus, R., Hirst, J., Bhumbra, G. S., Kozik, P., Jackson, L. P., Sahlender, D. A. and Robinson, M. S. Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles. J. Cell Biol. 197, 141–160 (2012).

Hirst, J. et al. Distinct and overlapping roles for AP-1 and GGAs revealed by the 'knocksideways' system. Curr. Biol. 22, 1711–1716 (2012).

Miller, S.E., Sahlender, D.A., Graham, S. C., Höning, S., Robinson, M. S., Peden, A. A. and Owen, D. J. The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Cell 147, 1118–1131 (2011).

Hirst, J., Francisco, G.C., Sahlender, D.A., Seaman, M.N.J., Dacks, J.B. and Robinson, M. S. The fifth adaptor protein complex. PLoS Biol. 9, e1001170 (2011).

Robinson, M.S.*, Sahlender, D. A. and Foster, S. D. Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria. Dev. Cell 18, 324–331 (2010).

References

Sedwick, Caitlin. “Margaret Robinson: Vesicles Wear Fancy Coats.” The Journal of Cell Biology 206.6 (2014): 692–693. PMC. Web. 14 Mar. 2016.

Robinson, Margaret S. "Margaret S Robinson." ResearchGate. University of Cambridge, 21 Aug. 2008. Web. 14 Mar. 2016.

Gray, Kate Arkless. "Researcher Spotlight: Professor Margaret Robinson." Wellcome Trust Blog. WordPress.com, 14 July 2014. Web. 14 Mar. 2016.

Royal Society Editors. "Margaret Robinson." Margaret Robinson. The Royal Society, 8 May 2012. Web. 14 Mar. 2016.

RNAi Global Editors. "Margaret Robinson." Margaret Robinson. RNAi Global, 11 Sept. 2006. Web. 14 Mar. 2016

Cambridge Institute for Medical Research Editors. "Margaret Robinson." Margaret S. Robinson. Cambridge Institute for Medical Research, 18 Feb. 2014. Web. 14 Mar. 2016. Tylerdeeh (talk) 16:00, 30 March 2016 (UTC)