Talk:Oocyte activation

added content
The following content was added in this dif. This appears to be important to include, but needs to be worked over for sourcing. The content is probably workable but it is written like a literature review, not a like an encyclopedia article. Will work on this; others are invited to as well. There are also claims about human health here that we need to be sure are sourced per WP:MEDRS.

Since its discovery in 2002, phospholipase C zeta (PLCζ) has been assumed to be the sperm factor responsible for triggering Ca2+ oscillations in the oocyte during its activation. PLC-δ4 knockout (KO) mice exhibit male infertility, and studies have shown that sperm from these animals fails to induce oocyte activation and to cause calcium oscillations.

Studies using recombinant PLC-ζ protein showed that this proteins by itself is capable to induce calcium oscillation and activation of the oocyte in mice. One of the animal models used to investigate the roles of PLC-ζ was a transgenic mouse that shows broad ectopic PLC-ζ expression. In these animals, PLC-ζ was ectopically expressed in their oocytes and although they initially appear healthy, their oocytes exhibit autonomous Ca2+ oscillation, second polar body extrusion, pronucleus formation, parthenogenetic development and some mice also developed benign ovarian teratomas.

PLC-ζ is present in both human and mice sperm, and it is assumed to have the same role in egg activation in both species. PLC-ζ was undetectable in sperm from patients who failed intracytoplasmic sperm injection (ICSI) and this same sperm were unable to induce Ca2+ oscillations in mouse oocytes. Otherwise when the sperm was injected with mouse PLC-ζ mRNA these patients restored the capacity of activate oocytes.

-- Jytdog (talk) 06:51, 8 December 2016 (UTC)

How long time
How long time does the process take for a human egg? — Preceding unsigned comment added by 183.89.196.156 (talk) 23:08, 26 January 2017 (UTC)