2000 US Open – Women's singles qualifying

This article displays the qualifying draw for the Women's singles at the 2000 US Open.

Seeds
• # 🇨🇿 Dája Bedáňová (qualified)

• # Sandra Načuk (qualifying competition, lucky loser)

• # 🇧🇾 Nadejda Ostrovskaya (first round)

• # 🇨🇦 Vanessa Webb (qualified)

• # 🇦🇺 Alicia Molik (qualifying competition, lucky loser)

• # 🇮🇹 Francesca Schiavone (qualified)

• # 🇩🇪 Marlene Weingärtner (first round, retired)

• # 🇪🇸 Nuria Llagostera Vives (first round)

• # Wynne Prakusya (first round)

• # 🇫🇷 Émilie Loit (second round)

• # 🇮🇹 Gloria Pizzichini (qualified)

• # 🇭🇺 Petra Mandula (qualified)

• # 🇧🇾 Tatiana Poutchek (qualifying competition)

• # 🇨🇦 Maureen Drake (first round)

• # Janet Lee (qualified)

• # 🇨🇳 Li Na (second round)

• # 🇩🇪 Barbara Rittner (second round)

• # 🇦🇺 Rachel McQuillan (qualified)

• # Yvette Basting (qualifying competition)

• # 🇬🇧 Louise Latimer (qualifying competition)

• # 🇸🇰 Martina Suchá (qualifying competition)

• # 🇯🇵 Yuka Yoshida (first round)

• # 🇧🇪 Laurence Courtois (qualifying competition)

• # 🇬🇧 Julie Pullin (second round)

• # 🇩🇪 Bianka Lamade (second round)

• # 🇦🇷 Mariana Díaz Oliva (first round)

• # Seda Noorlander (first round)

• # 🇪🇸 Gisela Riera (second round)

• # 🇨🇴 Catalina Castaño (first round)

• # Tina Križan (first round)

• # 🇮🇹 Francesca Lubiani (qualifying competition)

• # Eva Dyrberg (second round)

Qualifiers
• # 🇨🇿 Dája Bedáňová

• # Mirjana Lučić

• # 🇨🇿 Alena Vašková

• # 🇨🇦 Vanessa Webb

• # 🇩🇪 Angelika Bachmann

• # 🇮🇹 Francesca Schiavone

• # 🇹🇳 Selima Sfar

• # 🇭🇺 Kira Nagy

• # 🇺🇸 Jill Craybas

• # 🇩🇪 Gréta Arn

• # 🇮🇹 Gloria Pizzichini

• # 🇭🇺 Petra Mandula

• # 🇦🇺 Rachel McQuillan

• # 🇩🇪 Miriam Schnitzer

• # Janet Lee

• # 🇸🇰 Ľudmila Cervanová

Lucky losers
• # Sandra Načuk

• # 🇦🇺 Alicia Molik