2009 US Open – Women's singles qualifying

Seeds
• # 🇮🇹 Alberta Brianti (moved to main draw)

• # 🇬🇧 Elena Baltacha (qualifying competition)

• # 🇬🇧 Katie O'Brien (second round)

• # 🇨🇿 Sandra Záhlavová (second round)

• # 🇩🇪 Angelique Kerber (qualified)

• # Chan Yung-jan (qualifying competition)

• # 🇸🇰 Kristína Kučová (first round)

• # 🇺🇿 Akgul Amanmuradova (first round; retired)

• # 🇫🇷 Pauline Parmentier (qualifying competition)

• # 🇪🇸 Arantxa Parra Santonja (qualifying competition)

• # 🇷🇺 Ekaterina Bychkova (second round)

• # 🇨🇿 Barbora Záhlavová-Strýcová (qualified)

• # 🇨🇿 Klára Zakopalová (qualifying competition)

• # Karolina Šprem (second round)

• # 🇩🇪 Julia Schruff (qualifying competition)

• # 🇦🇹 Yvonne Meusburger (qualified)

• # 🇷🇺 Vitalia Diatchenko (second round)

• # 🇷🇺 Vesna Manasieva (qualified)

• # 🇩🇪 Kathrin Wörle (first round)

• # 🇺🇦 Mariya Koryttseva (qualified)

• # 🇫🇷 Stéphanie Foretz (first round)

• # 🇰🇿 Sesil Karatantcheva (second round)

• # 🇫🇷 Camille Pin (qualified)

• # 🇺🇸 Shenay Perry (qualified)

• # 🇺🇸 Carly Gullickson (qualified)

• # 🇺🇸 Julie Ditty (first round)

• # 🇺🇸 Angela Haynes (qualifying competition)

• # 🇷🇺 Ekaterina Ivanova (first round)

• # Petra Martić (qualified)

• # 🇦🇺 Anastasia Rodionova (qualified)

• # 🇨🇴 Mariana Duque Mariño (qualifying competition)

• # 🇵🇱 Marta Domachowska (qualified)

Qualifiers
• # Chang Kai-chen

• # 🇦🇺 Anastasia Rodionova

• # 🇯🇵 Yurika Sema

• # 🇵🇱 Marta Domachowska

• # 🇩🇪 Angelique Kerber

• # 🇦🇺 Monique Adamczak

• # 🇨🇿 Eva Hrdinová

• # Petra Martić

• # 🇺🇸 Shenay Perry

• # 🇷🇺 Vesna Manasieva

• # 🇺🇸 Carly Gullickson

• # 🇨🇿 Barbora Záhlavová-Strýcová

• # 🇺🇦 Mariya Koryttseva

• # 🇨🇦 Valérie Tétreault

• # 🇫🇷 Camille Pin

• # 🇦🇹 Yvonne Meusburger