2016 NCAA Division I women's soccer season

The 2016 NCAA Division I women's soccer season was the 35th season of NCAA championship women's college soccer. The Penn State Nittany Lions were the defending national champions.

Final polls
Note: Soccer America released its final poll on November 7, 2016, before the NCAA Tournament

Major upsets
Games won by a team ranked 10 or more spots lower or an unranked squad that defeats a team ranked #15 or higher are considered "major upsets". Only NSCAA rankings are used for this list.