2016 San Luis Open Challenger Tour – Singles

Guido Pella was the defending champion, but chose not to defend his title.

Peđa Krstin won the title, defeating Marcelo Arévalo 6–4, 6–2 in the final.

Seeds
• # 🇩🇪 Michael Berrer (first round)

• # 🇪🇸 Albert Montañés (semifinals)

• # 🇧🇷 André Ghem (quarterfinals)

• # 🇪🇸 Adrián Menéndez Maceiras (first round)

• # 🇮🇹 Matteo Donati (second round)

• # 🇫🇷 Mathias Bourgue (second round)

• # Hans Podlipnik (quarterfinals)

• # 🇮🇪 James McGee (first round)