2021 Kuchyně Gorenje Prague Open – Singles

Jana Čepelová was the defending champion but chose not to participate.

Magdalena Fręch won the title, defeating Tereza Smitková in the final, 6–2, 6–1.

Seeds
• # 🇵🇱 Magdalena Fręch (champion)

• # 🇵🇱 Katarzyna Kawa (second round)

• # 🇭🇺 Panna Udvardy (first round)

• # Leonie Küng (first round)

• # 🇮🇹 Lucia Bronzetti (second round)

• # 🇸🇰 Rebecca Šramková (quarterfinals)

• # 🇮🇹 Lucrezia Stefanini (first round)

• # 🇫🇷 Diane Parry (semifinals)