Talk:Luminol

I know lots of things can catalyse the breakdown of hydrogen peroxide, but surely red cell catalase is more important in this context than haemoglobin or any non-enzyme catalyst. They seem to think so at this site:

3-nitrophthalhydrazide or 5-nitrophthalhydrazide?
I thought the synthesis used 5-nitro, not 3-nitro. Not sure enough to change it though. — Preceding unsigned comment added by 140.180.187.50 (talk) 15:49, 10 April 2012 (UTC)

3-nitro is Ok, because the ring is open. After the ring closes, we must renumber the molecule and the same nitro group will be called 5-nitro. chami 17:51, 1 October 2012 (UTC) — Preceding unsigned comment added by Ck.mitra (talk • contribs)

Chemical compound components
looking at the structure on the page, it looks like there are only 2 oxygen atoms, yet the molecular formula listed has 3 oxygens listed -O3- I wanted to post this here for the original writer of the article without changing it myself in the case that I had missed something. Please and thanks for looking into this! —Preceding unsigned comment added by 99.240.111.89 (talk) 03:51, 6 October 2008 (UTC)
 * As far as I can see it looks like this has been fixed. I can't see O3 mentioned anywhere in the article and the content seems to be coherent with regards to the chemical formulas mentioned. ~  Fenrisulfr  ( talk  ·  work  ) 21:15, 29 April 2009 (UTC)

The description is not clear. I think all the heme containing proteins or enzymes will give a positive test with luminol, benzidine or tetramethylbenzidine. This is certainly true for horseradishperoxidase, catalase, hemoglobin and a host of other proteins. Oxygen alone is not enough and hydrogenperoxide reacts with catalase, horseradishperoxidase or hemeglobin and produces the oxidised enzyme. This in turn oxidises the dye, i.e., luminol, benzidine etc. and gives rise to the color. Although the test is specific for blood, other enzymes will also gives the test. Also, bleach is not a clear compound, you should tell whether it is active chlorine. Hydrogen peroxide is also called bleach and loosely speaking bleach is any decolourising chemical or biochemical reagent. Many detergents contain some proteolytic enzymes that they are often (wrongly) called bleaching agents. chami 18:38, 7 October 2011 (UTC) — Preceding unsigned comment added by Ck.mitra (talk • contribs)

History
When was Luminol first discovered, how was it first discovered, and who synthesized it first. If this article is to be comprehensive, that should definitely be in there. — Preceding unsigned comment added by 76.217.12.210 (talk) 02:55, 2 August 2012 (UTC)

Note on history: "In 1939, San Francisco pathologists Frederick Proescher and A. M. Moody made three important observations about luminol" - however, all those observations (and more) had already been made in the earlier paper by Walter Specht:

• 1. large areas of suspected material can be examined rapidly: "...dass auch ein ausgedehnter Tatort oder ein grosses Überfübrungsstück mit Schnelligkeit ohne Matarialverlust auf etwa vorhandene Blutspuren überprüft werden können." (..that also extended crime scenes or a large piece of evidence can be quickly tested for blood stains without loss of material."

• 2. dried and decomposed blood gave a stronger and more lasting reaction than fresh blood: "Je älter die Blutspur ist, um so deutlicher ist der Lichteffekt." (The older the blood trace is, the more distinct is the light effect"). Specht's paper made it clear that the method is very suitable for older traces.

• 3. it may be reproduced by the application of a fresh luminol-hydrogen peroxide solution. dried bloodstains may thus be made luminescent repeatedly: "Die Leuchtdauer betrug durchschnittlich 15 min. Nach erfolgter Reaktion konnte der Lichteffekt durch abermaliges Bespritzen mit der Versuchslösung widerum ausgelöst werden." (The duration of light emission was on average 15 min. After completion of the reaction, the light effect could again be induced by spraying with the reagent).

Furthermore he already recommended taking photographs to store the information (with examples).

So the "San Francisco pathologists" basically just repeated the work of Specht and published it in English...

Furthermore on the history of discovery of Luminol chemoluminescence: The 1928 Albrecht paper is the first describing the chemoluminescence of Luminol in the literature. He got the topic from Hans Krausky, to whom it was told by the original discoverer, Wilhelm Lommel, Industrial Chemist at IG Farben. This information is mentioned in the 1928 Albrecht article, and also in a 1936 article by Gleu (J. Prakt Chem. 1936, 137).92.194.10.170 (talk) 10:39, 28 May 2018 (UTC)

The Request for Sources
The "Technical Note" reference listed in in the links includes a thorough testing of standard aqueous luminol vs. a commercial preparation, and has an extensive list of the other substances that produced false positives when using luminol to locate blood stains. This could be used to improve the flagged sentences, but it is too long to be included entire; I am not certain what appropriate use would be. Nightsmaiden (talk) 07:57, 29 July 2009 (UTC)

Medical use
See http://www.sciencedaily.com/releases/2011/07/110720091538.htm -- Quick Test to Diagnose Bacterial or Viral Infection -- Jo3sampl (talk) 22:23, 6 September 2011 (UTC)

Error in the diagram
The diagram for the mechanism of luminol (excited triplet state) is incorrect as there is an extra double bond connected to the carbon. The correct mechanism is the same but with carbon having four bonds instead of five. source: http://pubs.acs.org/doi/abs/10.1021/ja01059a051 — Preceding unsigned comment added by 129.171.233.72 (talk) 01:47, 19 April 2013 (UTC)
 * confirm typo : should be corrected, concerns File:Luminol chemiluminescence molecular representation.svg molecule no 4. V8rik (talk) 18:45, 16 August 2013 (UTC)

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3-nitrophthalhydrazide
What is meant by But 3-nitrophthalhydrazide itself has the glow.? That it has a glow in addition to luminol? Or that it is in fact what glows? That makes no sense to me since it is involved in the synthesis not the luminescence. Pariah24 (talk) 02:16, 21 May 2020 (UTC)