2016 Australian Open – Boys' singles

Oliver Anderson won the title, defeating Jurabek Karimov in the final, 6–2, 1–6, 6–1.

Roman Safiullin was the defending champion, but was no longer eligible to participate in junior events.

Seeds
• 🇭🇺 Máté Valkusz (third round)

• 🇷🇸 Miomir Kecmanović (third round)

• Stefanos Tsitsipas (quarterfinals)

• 🇨🇦 Félix Auger-Aliassime (third round)

• 🇰🇷 Chung Yun-seong (semifinals)

• 🇦🇺 Alex De Minaur (semifinals)

• 🇺🇿 Jurabek Karimov (final)

• 🇺🇸 Ulises Blanch (second round)

• 🇬🇧 Jay Clarke (first round)

•  Alberto Lim (first round)

• 🇦🇷 Genaro Alberto Olivieri (second round)

• 🇪🇬 Youssef Hossam (third round)

• 🇯🇵 Yosuke Watanuki (quarterfinals)

• 🇫🇷 Ugo Humbert (second round)

• 🇪🇪 Kenneth Raisma (quarterfinals)

• 🇨🇳 Wu Yibing (first round)

Seeds
• # 🇺🇸 Ezekiel Clark (qualified)

• # 🇧🇪 Seppe Cuypers (qualified)

• # 🇭🇺 Zsombor Piros (qualified)

• # 🇩🇪 Rudolf Molleker (qualifying competition; lucky loser)

• # 🇮🇹 Andres Gabriel Ciurletti (qualified)

• # 🇷🇺 Mikhail Sokolovskiy (qualifying competition)

• # 🇯🇵 Rio Noguchi (qualified)

• # Bart Stevens (first round)

• # Karlo Boljat (qualifying competition)

• # 🇮🇹 Riccardo Balzerani (qualifying competition)

• # 🇦🇺 Kody Pearson (first round)

• # 🇨🇳 Cui Jie (qualifying competition)

• # 🇭🇰 Anthony Jackie Tang (qualified)

• # 🇪🇪 Kristofer Siimar (qualifying competition)

• # 🇮🇳 Nikshep Ballekere Ravikumar (first round)

• # 🇮🇹 Giovanni Fonio (qualifying competition)

Qualifiers
• # 🇺🇸 Ezekiel Clark

• # 🇧🇪 Seppe Cuypers

• # 🇭🇺 Zsombor Piros

• # 🇭🇰 Anthony Jackie Tang

• # 🇮🇹 Andres Gabriel Ciurletti

• # 🇯🇵 Daisuke Sumizawa

• # 🇯🇵 Rio Noguchi

• # 🇦🇺 Lucas Vuradin

Lucky losers
• # 🇩🇪 Rudolf Molleker