2016 Australian Open – Men's singles

Defending champion Novak Djokovic defeated Andy Murray in a rematch of the previous year's final, 6–1, 7–5, 7–6(7–3) to win the men's singles tennis title at the 2016 Australian Open. It was his record-equaling sixth Australian Open men's singles title (tying Roy Emerson) and eleventh major title overall. This was the fourth Australian Open final between the pair. Murray became the second man in the Open Era to lose five finals at the same major (after Ivan Lendl at the US Open), and is the only one to have the distinction without having won the title.

Australian Lleyton Hewitt, a former finalist, two-time major champion and former world No. 1, played his last professional singles match at this event. He lost to David Ferrer in the second round.

Seeds
• 🇷🇸 Novak Djokovic (champion)

• 🇬🇧 Andy Murray (final)

• Roger Federer (semifinals)

• Stan Wawrinka (fourth round)

• 🇪🇸 Rafael Nadal (first round)

• 🇨🇿 Tomáš Berdych (quarterfinals)

• 🇯🇵 Kei Nishikori (quarterfinals)

• 🇪🇸 David Ferrer (quarterfinals)

• 🇫🇷 Jo-Wilfried Tsonga (fourth round)

• 🇺🇸 John Isner (fourth round)

• Kevin Anderson (first round, retired)

• Marin Čilić (third round)

• 🇨🇦 Milos Raonic (semifinals)

• 🇫🇷 Gilles Simon (fourth round)

• 🇧🇪 David Goffin (fourth round)

• 🇦🇺 Bernard Tomic (fourth round)

• 🇫🇷 Benoît Paire (first round)

• 🇪🇸 Feliciano López (third round)

• 🇦🇹 Dominic Thiem (third round)

• 🇮🇹 Fabio Fognini (first round)

• 🇷🇸 Viktor Troicki (third round)

• Ivo Karlović (first round, retired)

• 🇫🇷 Gaël Monfils (quarterfinals)

• 🇪🇸 Roberto Bautista Agut (fourth round)

• 🇺🇸 Jack Sock (second round)

• 🇪🇸 Guillermo García-López (third round)

• 🇧🇬 Grigor Dimitrov (third round)

• 🇮🇹 Andreas Seppi (third round)

• 🇦🇺 Nick Kyrgios (third round)

• 🇫🇷 Jérémy Chardy (second round)

• 🇺🇸 Steve Johnson (third round)

• João Sousa (third round)