User:Yohhans

=BRTP 822=

Lu

 * 1) Mechanism of Transcription
 * 2) Transcription
 * 3) Structure of RNA
 * 4) RNA Synthesis
 * 5) Three stages in transcription
 * 6) RNA polymerase
 * 7) E. coli RNA polymerase
 * 8) Sigma factors
 * 9) Lac operon
 * 10) Control of elongation in E. coli
 * 11) Eukaryotic RNA polymerases
 * 12) Three Eukaryotic RNA polymerases are multi-protein complexes that share many common subunits
 * 13) Promoter
 * 14) TATA box
 * 15) Initiator sequences (INR)
 * 16) CpG island promoter
 * 17) General transcription factors
 * 18) Transcription initiation
 * 19) Regulation of transcription elongation

Trujillo

 * 1) Part 1
 * 2) Part 2
 * 3) Part 3
 * 4) Part 4

Bakhat
Misc. intro stuff
 * 1) Central problem is  to figure out how genes are selected for expression during growth, differentiation, or in response to the environment
 * 2) Need to determine how a gene is specified by cis-DNA
 * 3) cis-elements are promoters, enhancers, silencers and insulators

Gene regulation
First we are going to look at core promoter elements. The core promoter is a region that is upstream of the transcription start site (TSS +1), and it contains several elements that facilitate the binding of regulatory elements. These elements form the pre-initiation complex (PIC).

Core promoters are divided into two different classes: TSS is focused over a narrow region, or it is dispersed over a broad region (50-100bp). Some core promoter elements include BRE (GC rich region that is the TFIIB recognition site. CpG dinucleotide is very important here: [GC][GC][GA]CGCC, the TATA box (TATA[AT]A[AT][AG]), and the Inr. A promoter element downstream of the TSS is called a downstream core promoter element.

Despite the TATA box being the

Wang
=Old=