2000 Cincinnati Masters – Singles

Thomas Enqvist defeated Tim Henman in the final, 7–6(7–5), 6–4 to win the singles tennis title at the 2000 Cincinnati Masters.

Pete Sampras was the defending champion, but lost in the third round to Henman.

Seeds

 * 1) 🇺🇸 Andre Agassi (second round, retired)
 * 2) 🇺🇸 Pete Sampras (third round)
 * 3) 🇸🇪 Magnus Norman (second round)
 * 4) 🇧🇷 Gustavo Kuerten (semifinals)
 * 5) 🇷🇺 Yevgeny Kafelnikov (third round)
 * 6) 🇪🇸 Álex Corretja (first round)
 * 7) 🇸🇪 Thomas Enqvist (champion)
 * 8) 🇷🇺 Marat Safin (third round)
 * 9) 🇦🇺 Lleyton Hewitt (first round)
 * 10) 🇪🇨 Nicolás Lapentti (first round)
 * 11) 🇩🇪 Nicolas Kiefer (first round)
 * 12) 🇪🇸 Juan Carlos Ferrero (first round)
 * 13) 🇦🇷 Franco Squillari (quarterfinals)
 * 14) 🇲🇦 Younes El Aynaoui (withdrew)
 * 15) 🇬🇧 Tim Henman (final)
 * 16) 🇦🇺 Mark Philippoussis (third round)

Qualifying seeds
• # 🇮🇹 Gianluca Pozzi (qualified)

• # 🇧🇾 Vladimir Voltchkov (qualified)

• # 🇨🇿 Martin Damm (qualified)

• # George Bastl (first round)

• # 🇮🇹 Davide Sanguinetti (first round)

• # Peter Wessels (first round)

• # 🇮🇹 Andrea Gaudenzi (qualifying competition, lucky loser)''

• # 🇫🇷 Cyril Saulnier (first round)

• # 'Cecil Mamiit (qualified)''

• # 🇺🇸 Bob Bryan (qualified)

• # 🇸🇰 Ján Krošlák (first round)

• # Fernando González (qualifying competition)

• # Kevin Ullyett (first round, retired due to a knee injury)

• # 🇬🇧 Arvind Parmar (qualifying competition)

• # 🇫🇷 Jean-René Lisnard (first round)

• # Marcos Ondruska (qualifying competition)

Qualifiers
• # 🇮🇹 Gianluca Pozzi

• # 🇧🇾 Vladimir Voltchkov

• # 🇨🇿 Martin Damm

• # Neville Godwin

• # 🇺🇸 Taylor Dent

• # 🇫🇷 Lionel Roux

• # Cecil Mamiit

• # 🇺🇸 Bob Bryan

Lucky loser

 * 1) 🇮🇹 Andrea Gaudenzi

Special exempt

 * 1) 🇮🇱 Harel Levy (runner-up at Toronto)