2000 Australian Open – Mixed doubles

Rennae Stubbs and Jared Palmer defeated 1993 champions Arantxa Sánchez Vicario and Todd Woodbridge in the final, 7–5, 7–6(7–3), to win the mixed doubles tennis title at the 2000 Australian Open. By winning the title, Stubbs won her second Grand Slam title, after she won her first in Women's Doubles earlier in the week, and Palmer won his first major Mixed Doubles title. Mariaan de Swardt and David Adams were the defending champions, but De Swardt chose not to defend her title and Adams played with Kristie Boogert. Adams and Boogert lost to Sánchez Vicario and Woodbridge in the semifinals.

Seeds
Champion seeds are indicated in bold text while text in italics indicates the round in which those seeds were eliminated.

• # 🇷🇺 Anna Kournikova / 🇸🇪 Jonas Björkman (semifinals)

• # 🇺🇸 Lisa Raymond / 🇮🇳 Leander Paes (first round)

• # 🇦🇺 Rennae Stubbs / 🇺🇸 Jared Palmer (champions)

• # 🇪🇸 Arantxa Sánchez Vicario / 🇦🇺 Todd Woodbridge (final)

• # 🇧🇾 Natasha Zvereva / 🇦🇺 Mark Woodforde (quarterfinals)

• # 🇷🇺 Elena Likhovtseva / 🇺🇸 Jeff Tarango (quarterfinals)

• # Caroline Vis / John-Laffnie de Jager (second round)

• # 🇺🇦 Elena Tatarkova / 🇷🇺 Andrei Olhovskiy (quarterfinals)