2009 Mutua Madrileña Madrid Open – Women's singles

Dinara Safina defeated Caroline Wozniacki in the final, 6–2, 6–4 to win the inaugural women's singles tennis title at the 2009 Madrid Open.

Seeds
• # 🇷🇺 Dinara Safina (champion)

• # 🇺🇸 Serena Williams (first round, retired because of a knee injury)

• # 🇷🇺 Elena Dementieva (third round)

• # 🇷🇸 Jelena Janković (quarterfinals)

• # 🇺🇸 Venus Williams (second round)

• # 🇷🇺 Svetlana Kuznetsova (second round)

• # 🇧🇾 Victoria Azarenka (third round)

• # 🇷🇺 Nadia Petrova (third round)

• # Caroline Wozniacki (final)

• # 🇵🇱 Agnieszka Radwańska (first round)

• # 🇫🇷 Marion Bartoli (first round)

• # 🇮🇹 Flavia Pennetta (first round)

• # 🇫🇷 Alizé Cornet (first round)

• # 🇪🇸 Anabel Medina Garrigues (first round)

• # 🇨🇳 Zheng Jie (second round)

• # 🇪🇪 Kaia Kanepi (first round, retired)

The four Rome semifinalists received a bye into the second round. They were as follows:
 * 🇷🇺 Dinara Safina (champion)
 * 🇺🇸 Venus Williams (second round)
 * 🇷🇺 Svetlana Kuznetsova (second round)
 * 🇧🇾 Victoria Azarenka (third round)