2009 Italian Open – Women's singles

Dinara Safina defeated Svetlana Kuznetsova in the final, 6–3, 6–2 to win the women's singles tennis title at the 2009 Italian Open.

Jelena Janković was the two-time defending champion, but lost in the quarterfinals to Kuznetsova.

Seeds
The top eight seeds receive a bye into the second round. • # 🇷🇺 Dinara Safina (champion)

• # 🇺🇸 Serena Williams (second round)

• # 🇷🇸 Jelena Janković (quarterfinals)

• # 🇺🇸 Venus Williams (semifinals)

• # 🇷🇸 Ana Ivanovic (third round)

• # 🇧🇾 Victoria Azarenka (semifinals)

• # 🇷🇺 Svetlana Kuznetsova (final)

• # 🇷🇺 Nadia Petrova (third round)

• # Caroline Wozniacki (third round)

• # 🇵🇱 Agnieszka Radwańska (quarterfinals)

• # 🇫🇷 Marion Bartoli (second round)

• # 🇮🇹 Flavia Pennetta (third round)

• # 🇫🇷 Alizé Cornet (second round)

• # 🇨🇳 Zheng Jie (third round)

• # 🇪🇸 Anabel Medina Garrigues (first round)

• # 🇪🇪 Kaia Kanepi (quarterfinals)