2009 Western & Southern Financial Group Women's Open – Singles

Jelena Janković defeated Dinara Safina in the final, 6–4, 6–2 to win the women's singles tennis title at the 2009 Cincinnati Masters.

Nadia Petrova was the defending champion, but lost in the first round to Alona Bondarenko.

This tournament marked the return of Kim Clijsters from the sport after her first retirement, having not played a match since May 2007. She advanced to the quarterfinals, where she was beaten by Safina.

Seeds
The top eight seeds receive a bye into the second round.


 * 1) 🇷🇺 Dinara Safina (final)
 * 2) 🇺🇸 Serena Williams (third round)
 * 3) 🇺🇸 Venus Williams (third round)
 * 4) 🇷🇺 Elena Dementieva (semifinals)
 * 5) 🇷🇸 Jelena Janković (champion)
 * 6) 🇷🇺 Svetlana Kuznetsova (third round)
 * 7) 🇷🇺 Vera Zvonareva (third round)
 * 8) Caroline Wozniacki (quarterfinals)
 * 9) 🇧🇾 Victoria Azarenka (third round)
 * 10) 🇷🇺 Nadia Petrova (first round)
 * 11) 🇷🇸 Ana Ivanovic (second round)
 * 12) 🇫🇷 Marion Bartoli (first round)
 * 13) 🇵🇱 Agnieszka Radwańska (second round)
 * 14) 🇮🇹 Flavia Pennetta (semifinals)
 * 15) 🇸🇰 Dominika Cibulková (first round)
 * 16) 🇫🇷 Virginie Razzano (first round)