2021 Belgrade Challenger – Women's singles

This was the first edition of the tournament.

Anna Karolína Schmiedlová won the title, defeating Arantxa Rus in the final, 6–3, 6–3.

Seeds
• # 🇷🇺 Anna Blinkova (quarterfinals)

• # Arantxa Rus (final)

• # 🇷🇺 Varvara Gracheva (second round)

• # 🇩🇪 Andrea Petkovic (semifinals)

• # 🇮🇹 Martina Trevisan (quarterfinals)

• # Kaja Juvan (first round)

• # 🇷🇴 Irina Bara (second round)

• # 🇧🇬 Viktoriya Tomova (first round)

Seeds
• # 🇫🇷 Jessika Ponchet (qualified)

• # 🇧🇾 Shalimar Talbi (qualifying competition)

• # 🇸🇰 Zuzana Zlochová (qualifying competition)

• # 🇮🇹 Camilla Rosatello (qualified)

Qualifiers
• # 🇫🇷 Jessika Ponchet

• # 🇷🇺 Ekaterina Yashina

• # Tara Würth

• # 🇮🇹 Camilla Rosatello