2021 Columbus Challenger – Women's singles

This was the first edition of the women's event.

Nuria Párrizas Díaz won the title, defeating Wang Xinyu in the final, 7–6(7–2), 6–3.

Seeds
• # 🇺🇸 Ann Li (quarterfinals)

• # 🇪🇸 Nuria Párrizas Díaz (champion)

• # 🇺🇸 Madison Brengle (quarterfinals)

• # 🇨🇳 Zheng Saisai (semifinals)

• # 🇺🇸 Lauren Davis (quarterfinals)

• # 🇲🇽 Renata Zarazúa (second round)

• # 🇧🇷 Beatriz Haddad Maia (quarterfinals)

• # 🇨🇳 Wang Xinyu (final)

Seeds
• # 🇦🇺 Priscilla Hon (qualified)

• # 🇯🇵 Mai Hontama (qualifying competition)

• # 🇺🇸 Alycia Parks (first round)

• # 🇺🇸 Whitney Osuigwe (first round)

• # 🇺🇸 Danielle Lao (qualified)

• # Dalila Jakupović (qualifying competition, lucky loser)

• # 🇺🇸 Alexa Glatch (qualified)

• # 🇳🇴 Ulrikke Eikeri (qualifying competition)

Qualifiers
• # 🇦🇺 Priscilla Hon

• # 🇺🇸 Alexa Glatch

• # 🇺🇸 Louisa Chirico

• # 🇺🇸 Danielle Lao

Lucky loser
• # Dalila Jakupović